Bagheri Seyyed Majid, Maghsoudi Mohammad Javad, Yadegari Maryam
Department of Physiology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Neurobiomedical Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Int J Prev Med. 2020 Nov 26;11:179. doi: 10.4103/ijpvm.IJPVM_108_19. eCollection 2020.
is introduced as a valuable remedy for hysteria and some other nervous disorders in Iranian traditional medicine. Asafoetida is an oleo-gum-resin obtained from the exudates of the roots of the . Previous studies have shown that this oleo gum resin has antioxidant, anti-apoptosis, and differentiation properties in the nervous system. The aim of this study was to evaluate the effect of asafoetida on the death of oligodendrocytes and demyelination in male C57BL/6 mice in cuprizone (CPZ)-induced animal model of multiple sclerosis.
Demyelination was induced by oral administration of rats with the 0.2% CPZ that was added to the usual diet for 8 weeks. Animals intraperitoneally received daily asafoetida at doses of 25 or 50 mg/kg of bodyweight simultaneously. At the end of the weeks, animal brains were removed and fixed to histological studies using Luxol fast blue staining. Asafoetida was screened for its antioxidant activity using 2, 2-diphenyl-1-picylhydrazyl free radical scavenging assay and for its inhibitory activity against lipid peroxidation catalyzed by soybean lipoxygenase.
The results of this study showed that asafoetida significantly decreased infiltration rate in both groups of asafoetida 25 and 50 mg/kg, respectively ( < 0.01). Histological evaluations showed the lower demyelination in LFB in the group treated with asafoetida.
The results of this study showed that asafoetida plays a neuro protective role in CPZ models of multiple sclerosis by reducing neuronal demyelination and oligodendrocytes death.
在伊朗传统医学中，阿魏作为治疗癔症和其他一些神经紊乱的有效药物被引入。阿魏是从植物根部渗出物中获得的一种油胶树脂。先前的研究表明，这种油胶树脂在神经系统中具有抗氧化、抗凋亡和分化特性。本研究的目的是评估阿魏对用铜螯合剂（CPZ）诱导的雄性C57BL/6小鼠多发性硬化动物模型中少突胶质细胞死亡和脱髓鞘的影响。
通过给大鼠口服添加到常规饮食中的0.2% CPZ诱导脱髓鞘8周。动物腹腔内每天同时接受25或50毫克/千克体重剂量的阿魏。在实验周期结束时，取出动物大脑并使用卢氏固蓝染色进行组织学研究。使用2,2-二苯基-1-苦基肼自由基清除试验筛选阿魏的抗氧化活性，并检测其对大豆脂氧合酶催化的脂质过氧化的抑制活性。
本研究结果表明，阿魏在25毫克/千克和50毫克/千克剂量组中均显著降低了浸润率（<0.01）。组织学评估显示，阿魏治疗组的卢氏固蓝染色脱髓鞘程度较低。
本研究结果表明，阿魏通过减少神经元脱髓鞘和少突胶质细胞死亡，在CPZ诱导的多发性硬化模型中发挥神经保护作用。
