Graduate School of Life Science, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.
Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan ; Present address: Faculty of Medicine, Shimane University, Izumo, Shimane 693-8501, Japan.
Biosci Microbiota Food Health. 2012;31(3):67-70. doi: 10.12938/bmfh.31.67. Epub 2012 Jul 26.
We investigated Candida albicans-induced mast cell degranulation in vitro and in vivo. Cell wall fraction but not culture supernatant and cell membrane fraction prepared from hyphally grown C. albicans induced β-hexosaminidase release in RBL-2H3 cells. Cell wall mannan and soluble β-glucan fractions also induced β-hexosaminidase release. Histological examination of mouse forestomach showed that C. albicans gut colonization induces mast cell degranulation. However, intragastric administration of cell wall fraction failed to induce mast cell degranulation. We propose that cell wall polysaccharides are responsible for mast cell degranulation in the C. albicans-colonized gut.
我们研究了白念珠菌诱导的体外和体内肥大细胞脱颗粒。从菌丝生长的白念珠菌中制备的细胞壁部分而非培养上清液和细胞膜部分诱导 RBL-2H3 细胞中β-己糖胺酶释放。细胞壁甘露聚糖和可溶性β-葡聚糖部分也诱导β-己糖胺酶释放。对小鼠前胃组织学检查表明，白念珠菌肠道定植诱导肥大细胞脱颗粒。然而，胃内给予细胞壁部分未能诱导肥大细胞脱颗粒。我们提出，细胞壁多糖是白念珠菌定植肠道中肥大细胞脱颗粒的原因。
