Environmental Biotechnology Laboratory, Department of Civil Engineering, The University of Hong Kong SAR , China.
Environ Sci Technol. 2014 Jul 15;48(14):8044-52. doi: 10.1021/es5017087. Epub 2014 Jun 25.
The objective of this study was to investigate the microbial community shifts, especially nitrifiers and denitrifiers, in the biofilm of two rotating biological contactor (RBC) trains with different running times along the plug flowpath. The microbial consortia were profiled using multiple approaches, including 454 high-throughput sequencing of the V3-V4 region of 16S rRNA gene, clone libraries, and quantitative polymerase chain reaction (qPCR). The results demonstrated that (1) the overall microbial community at different locations had distinct patterns, that is, there were similar microbial communities at the beginnings of the two RBC trains and completely different populations at the ends of the two RBC trains; (2) nitrifiers, including ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB, Nitrosomonas) and nitrite-oxidizing bacteria (NOB, Nitrospira), increased in relative abundance in the biofilm along the flowpath, whereas denitrifiers (Rhodanobacter, Paracoccus, Thauera, and Azoarcus) markedly decreased; (3) the AOA were subdominant to the AOB in all sampled sections; and (4) strong ecological associations were shown among different bacteria. Overall, the results of this study provided more comprehensive information regarding the biofilm community composition and assemblies in full-scale RBCs.
本研究的目的是调查沿推流式两个不同运行时间的旋转生物接触器（RBC）列车生物膜中的微生物群落变化，特别是硝化细菌和反硝化细菌。采用多种方法对微生物群落进行了分析，包括 V3-V4 区 16S rRNA 基因的 454 高通量测序、克隆文库和定量聚合酶链反应（qPCR）。结果表明：（1）不同位置的整体微生物群落具有明显的模式，即在两个 RBC 列车的开头有相似的微生物群落，而在两个 RBC 列车的末尾则有完全不同的种群；（2）硝化细菌，包括氨氧化古菌（AOA）、氨氧化细菌（AOB、Nitrosomonas）和亚硝酸盐氧化细菌（NOB、Nitrospira），在生物膜中的相对丰度沿流程增加，而反硝化细菌（Rhodanobacter、Paracoccus、Thauera 和 Azoarcus）则明显减少；（3）在所有采样部分，AOA 均次于 AOB；（4）不同细菌之间存在强烈的生态关联。总体而言，本研究的结果提供了更全面的关于全尺寸 RBC 中生物膜群落组成和组装的信息。
