Xue Liang, Ren Huadong, Li Sheng, Leng Xiuhui, Yao Xiaohua
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, China.
Observation and Research Station for Rock Desert Ecosystem, Puding, China.
Front Microbiol. 2017 Dec 1;8:2377. doi: 10.3389/fmicb.2017.02377. eCollection 2017.
Vegetation restoration has been widely used in karst rocky desertification (KRD) areas of southwestern China, but the response of microbial community to revegetation has not been well characterized. We investigated the diversity, structure, and co-occurrence patterns of bacterial communities in soils of five vegetation types (grassland, shrubbery, secondary forest, pure plantation and mixed plantation) in KRD area using high-throughput sequencing of the 16S rRNA gene. Bray-Curtis dissimilarity analysis revealed that 15 bacterial community samples were clustered into five groups that corresponded very well to the five vegetation types. Shannon diversity was positively correlated with pH and Ca content but negatively correlated with organic carbon, total nitrogen, and soil moisture. Redundancy analysis indicated that soil pH, Ca content, organic carbon, total nitrogen, and soil moisture jointly influenced bacterial community structure. Co-occurrence network analysis revealed non-random assembly patterns of bacterial composition in the soils. , GR-WP33-30, and were identified as keystone genera in co-occurrence network. These results indicate that diverse soil physicochemical properties and potential interactions among taxa during vegetation restoration may jointly affect the bacterial community structure in KRD regions.
植被恢复已在中国西南喀斯特石漠化地区广泛应用，但微生物群落对植被恢复的响应尚未得到很好的描述。我们利用16S rRNA基因的高通量测序技术，研究了喀斯特石漠化地区五种植被类型（草地、灌木丛、次生林、纯林和混交林）土壤中细菌群落的多样性、结构和共现模式。Bray-Curtis差异分析表明，15个细菌群落样本聚为五组，与五种植被类型非常吻合。香农多样性与pH值和钙含量呈正相关，但与有机碳、总氮和土壤湿度呈负相关。冗余分析表明，土壤pH值、钙含量、有机碳、总氮和土壤湿度共同影响细菌群落结构。共现网络分析揭示了土壤中细菌组成的非随机组装模式。GR-WP33-30和被确定为共现网络中的关键属。这些结果表明，植被恢复过程中不同的土壤理化性质和类群间潜在的相互作用可能共同影响喀斯特石漠化地区的细菌群落结构。
