叠氮化钠浓度对水中肠球菌回收的影响。

The effect of sodium azide concentration on the recovery of enterococci from water.

机构信息

CRF Consulting Ltd, Child's Acre, Church Lane, Three Mile Cross, Reading, RG7 1HD, UK and School of Environmental Studies, Queen's University, Kingston, Ontario, Canada E-mail:

Analytical Services Inc., Allen Brook Lane, Williston, Vermont, USA.

出版信息

J Water Health. 2014 Jun;12(2):264-8. doi: 10.2166/wh.2013.422.

DOI:10.2166/wh.2013.422

PMID:24937220

Abstract

The ability of Slanetz and Bartley medium to recover chlorine-stressed enterococci has been studied. Results showed that chlorine injury significantly affected the ability of Slanetz and Bartley medium to recover enterococci while lower concentrations of sodium azide in the same basal medium allowed their recovery. However, reducing the concentration of sodium azide considerably reduced the specificity making it unsuitable for use in the routine examination of water. A non-azide-containing medium, Enterolert(®)-DW appeared to be able to recover injured and non-injured enterococci with similar efficiency. The data presented here suggest that further work is required to improve the recovery of chlorine-injured enterococci by Slanetz and Bartley medium.

摘要

已研究 Slanetz 和 Bartley 培养基回收氯应激肠球菌的能力。结果表明，氯损伤显著影响 Slanetz 和 Bartley 培养基回收肠球菌的能力，而同一基础培养基中较低浓度的叠氮化钠允许其恢复。然而，降低叠氮化钠的浓度会大大降低特异性，使其不适合用于水的常规检查。一种不含叠氮化钠的培养基 Enterolert(®)-DW 似乎能够以类似的效率回收受伤和未受伤的肠球菌。这里提出的数据表明，需要进一步研究以提高 Slanetz 和 Bartley 培养基对氯损伤肠球菌的回收率。