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使用斯兰尼茨-巴特利琼脂、KF链球菌琼脂和MUST方法对肠道肠球菌及干扰菌进行计数。

Enumeration of intestinal enterococci and interfering organisms with Slanetz-Bartley agar, KF streptococcus agar and the MUST method.

作者信息

Niemi R M, Ahtiainen J

机构信息

National Board of Waters and the Environment, Helsinki, Finland.

出版信息

Lett Appl Microbiol. 1995 Feb;20(2):92-7. doi: 10.1111/j.1472-765x.1995.tb01294.x.

DOI:10.1111/j.1472-765x.1995.tb01294.x
PMID:7765907
Abstract

The recovery of intestinal species of enterococci and streptococci and potentially interfering nonfaecal species was measured on KF streptococcus agar, Slanetz-Bartley agar and in a medium based on 4-methylumbelliferyl-beta-D-glucoside (MUST method) using pure cultures. Both of the solid media yielded high recoveries of the target species. Their selectivity was better at elevated incubation temperature but nonfaecal Enterococcus and Staphylococcus species were not eliminated even at the elevated temperature. The MUST method tended to give slightly lower recoveries than the agar cultivation methods with some target species at 44 degrees C but recoveries were better at 41 degrees C.

摘要

使用纯培养物,在KF链球菌琼脂、Slanetz - Bartley琼脂以及基于4 - 甲基伞形酮基 - β - D - 葡萄糖苷的培养基(MUST法)上测定肠球菌和链球菌的肠道菌种以及可能产生干扰的非粪便菌种的回收率。两种固体培养基对目标菌种的回收率都很高。在较高培养温度下它们的选择性更好,但即使在较高温度下,非粪便肠球菌和葡萄球菌菌种也没有被消除。MUST法在44℃时对某些目标菌种的回收率往往比琼脂培养法略低,但在41℃时回收率更好。

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Enumeration of intestinal enterococci and interfering organisms with Slanetz-Bartley agar, KF streptococcus agar and the MUST method.使用斯兰尼茨-巴特利琼脂、KF链球菌琼脂和MUST方法对肠道肠球菌及干扰菌进行计数。
Lett Appl Microbiol. 1995 Feb;20(2):92-7. doi: 10.1111/j.1472-765x.1995.tb01294.x.
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