Akintola A O, Kehinde A O, Adebiyi O E, Ademowo O G
Department of Science Laboratory Technology, Ladoke Akintola University of Technology, Ogbomoso.
Niger J Physiol Sci. 2013 Dec 20;28(2):135-40.
Tuberculosis (TB) is of great public health burden globally especially in developing countries of Africa and Asia . Current TB regimen involves multiple therapies and of long duration leading to poor patient adherence. There is also the challenge of multidrug resistant TB. Hence, there is a need for discovery of new anti- TB drugs. This study was designed to investigate the in -vitro activity of the crude methanolic extract and chromatographic fractions of the bulb of Crinum jagus against Mycobacterium tuberculosis isolates. The extracts were screened for anti- TB activity against three different M. tuberculosis isolates and a drug susceptible reference strain H37Rv using Lowenstein Jensen (L-J) medium and Middlebrook 7H10agar. The crude extract was prepared using soxhlet extraction apparatus while the purified fractions were obtained by column chromatography. The two media were inoculated with M. tuberculosis strains, after which the crude and purified extracts were added. After 4-6 weeks incubation, colony forming units were counted and percentage inhibition calculated. The crude extract and the purified fractions showed inhibitory activity on all the isolates tested including the reference strain. Fraction 3 showed the highest inhibitory percentage (86%) among the extracts. At a concentration of 1.0mg/ml, the percentage inhibition of fraction 3, rifampicin and isoniazid against M. tuberculosis strain 3 were 83%, 95% and 86% in L-J medium respectively while 86%, 96% and 89% were obtained respectively in Middle brook medium. Results showed that the crude methanolic extract and the purified fractions of the bulb of Crinum jagus exhibited anti-mycobacterial activity which is an indication of promising potential of this plant for the development of anti-tuberculosis agent.
结核病在全球尤其是非洲和亚洲的发展中国家造成了巨大的公共卫生负担。目前的结核病治疗方案涉及多种疗法且疗程漫长，导致患者依从性差。此外，耐多药结核病也是一个挑战。因此，需要发现新的抗结核药物。本研究旨在调查文殊兰鳞茎的粗甲醇提取物及其色谱分离组分对结核分枝杆菌分离株的体外活性。使用罗氏培养基（L-J）和Middlebrook 7H10琼脂对提取物进行针对三种不同结核分枝杆菌分离株和一种药物敏感参考菌株H37Rv的抗结核活性筛选。粗提取物采用索氏提取器制备，而纯化组分通过柱色谱法获得。将结核分枝杆菌菌株接种到两种培养基中，然后加入粗提取物和纯化提取物。培养4 - 6周后，计数菌落形成单位并计算抑制百分比。粗提取物和纯化组分对所有测试的分离株包括参考菌株均显示出抑制活性。在提取物中，组分3显示出最高的抑制百分比（86%）。在浓度为1.0mg/ml时，组分3、利福平和平共处五项原则对结核分枝杆菌菌株3在罗氏培养基中的抑制百分比分别为83%、95%和86%，而在Middle brook培养基中分别为86%、96%和89%。结果表明，文殊兰鳞茎的粗甲醇提取物及其纯化组分具有抗分枝杆菌活性，这表明该植物在开发抗结核药物方面具有潜在的前景。
