Ajiwhen I O, Bisong S A
Department of Human Physiology, University of Calabar, Calabar.
Niger J Physiol Sci. 2013 Dec 20;28(2):141-5.
Carpolobia lutea, commonly called cattle stick or poor man's candle, is used by traditional herbalists in eastern Nigeria to treat 'madness'. It has a reported analgesic and anti-nociceptive effect. The effect of its ethanolic root extract on learning and memory was investigated. Thirty mice were divided into three groups of ten each. One group of mice served as the control and was given normal saline (p.o.) while the other two groups were given acute low dose (1500mg/kg, p.o.) and high dose (2500mg/kg, p.o.) (LD50 3338.83mg/kg). The effect of the extract on cognitive memory was investigated using the Novel Object recognition task (NORT) while the effect on visuospatial learning and memory was studied using the Morris Water maze (MWM). The results obtained in the NORT show that the index of habituation was significantly lower following acute treatment with a low dose of C. lutea extract compared to control. However, the index of habituation did not differ following treatment with a high dose of C. lutea compared to control but it was higher compared to the low dose. Following treatment with a low dose of the extract, the index of discrimination was significantly higher compared to control. The index of discrimination in the high dose treatment group did not differ from control, but it was lower compared to the low dose treatment. This indicated that there was improved cognitive memory only in the low dose treatment group. In the MWM there was no significant difference in swim latency during Acquisition and Reversal training. There also was no significant difference in quadrant duration during probe trial. The swim latency during the visible platform test showed that all mice used had good visual acuity. Therefore, the ethanolic extract of C. lutea root enhanced cognitive memory. However it did not affect visuospatial learning and memory.
黄果藤，通常被称为牛棍或穷人的蜡烛，尼日利亚东部的传统草药师用它来治疗“疯癫”。据报道，它具有镇痛和抗伤害感受的作用。研究了其乙醇根提取物对学习和记忆的影响。将30只小鼠分成三组，每组10只。一组小鼠作为对照组，口服生理盐水，而另外两组分别给予急性低剂量（1500mg/kg，口服）和高剂量（2500mg/kg，口服）（半数致死量为3338.83mg/kg）。使用新物体识别任务（NORT）研究提取物对认知记忆的影响，同时使用莫里斯水迷宫（MWM）研究对视觉空间学习和记忆的影响。在NORT中获得的结果表明，与对照组相比，低剂量黄果藤提取物急性处理后习惯化指数显著降低。然而，与对照组相比，高剂量黄果藤处理后习惯化指数没有差异，但与低剂量相比更高。低剂量提取物处理后，辨别指数与对照组相比显著更高。高剂量处理组的辨别指数与对照组没有差异，但与低剂量处理组相比更低。这表明只有低剂量处理组的认知记忆得到了改善。在MWM中，获取和反转训练期间的游泳潜伏期没有显著差异。在探测试验期间，象限持续时间也没有显著差异。可见平台测试期间的游泳潜伏期表明，所有使用的小鼠视力良好。因此，黄果藤根的乙醇提取物增强了认知记忆。然而，它不影响视觉空间学习和记忆。
