Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
Biochem Biophys Res Commun. 2014 Jul 18;450(1):318-23. doi: 10.1016/j.bbrc.2014.05.113. Epub 2014 Jun 2.
ELOVL family member 6, elongation of very long-chain fatty acids (Elovl6) is a microsomal enzyme that regulates the elongation of C12-16 saturated and monounsaturated fatty acids and is related to the development of obesity-induced insulin resistance via the modification of the fatty acid composition. In this study, we investigated the role of systemic Elovl6 in the pancreatic islet and β-cell function. Elovl6 is expressed in both islets and β-cell lines. In mice fed with chow, islets of the Elovl6(-/-) mice displayed normal architecture and β-cell mass compared with those of the wild-type mice. However, when fed a high-fat, high-sucrose (HFHS) diet, the islet hypertrophy in response to insulin resistance observed in normal mice was attenuated and glucose-stimulated insulin secretion (GSIS) increased in the islets of Elovl6(-/-) mice compared with those of the wild-type mice. Enhanced GSIS in the HFHS Elovl6(-/-) islets was associated with an increased ATP/ADP ratio and the suppression of ATF-3 expression. Our findings suggest that Elovl6 could be involved in insulin secretory capacity per β-cell and diabetes.
ELOVL 家族成员 6（Elovl6）是一种微粒体酶，可调节 C12-16 饱和和单不饱和脂肪酸的延伸，并且通过脂肪酸组成的修饰与肥胖诱导的胰岛素抵抗的发展有关。在这项研究中，我们研究了系统性 Elovl6 在胰岛和β细胞功能中的作用。Elovl6 在胰岛和β细胞系中均有表达。在食用标准饲料的小鼠中，与野生型小鼠相比，Elovl6（-/-）小鼠的胰岛显示出正常的结构和β细胞质量。然而，当喂食高脂肪、高蔗糖（HFHS）饮食时，正常小鼠中观察到的胰岛素抵抗引起的胰岛肥大反应减弱，并且与野生型小鼠相比，Elovl6（-/-）小鼠的胰岛中葡萄糖刺激的胰岛素分泌（GSIS）增加。HFHS Elovl6（-/-）胰岛中增强的 GSIS 与增加的 ATP/ADP 比值和 ATF-3 表达的抑制有关。我们的研究结果表明，Elovl6 可能参与β细胞的胰岛素分泌能力和糖尿病。
