Wen J-T, Zhou Y, Yang L, Xu Y
Department of Clinical Laboratory, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu Province, China.
Department of Clinical Laboratory, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu Province, China
Genet Mol Res. 2014 May 16;13(2):3842-9. doi: 10.4238/2014.May.16.9.
We examined the distribution of genes of aminoglycoside-modifying enzymes and 16S rRNA methylases in multidrug-resistant Acinetobacter baumannii to explore the association of these genes with drug resistance. Strains isolated from clinical specimens were screened using an automatic microbial identification system, and 9 aminoglycoside-modifying enzyme and 6 16S rRNA methylase genes were analyzed using polymerase chain reaction and verified by DNA sequencing. Next, sequence alignment was carried out using the Chromas software and a susceptibility test was performed using the Kirby-Bauer disk diffusion method. Genes encoding aminoglycoside-modifying enzymes were detected in all 20 strains of multidrug-resistant A. baumannii. The positive rates of aac(3')-I, aac(6')-Ib, ant(3'')-I, and aph(3')-I were 90.0, 90.0, 85.0, and 35.0%, respectively. However, genes encoding 16S rRNA methylases were not positively detected in the 20 strains of multidrug-resistant A. baumannii. The resistance of multidrug-resistant A. baumannii may be associated with aminoglycoside-modifying enzyme genes but not with 16S rRNA methylase genes.
我们检测了多重耐药鲍曼不动杆菌中氨基糖苷类修饰酶和16S rRNA甲基化酶基因的分布，以探讨这些基因与耐药性的相关性。使用自动微生物鉴定系统对从临床标本中分离出的菌株进行筛选，并采用聚合酶链反应分析9种氨基糖苷类修饰酶基因和6种16S rRNA甲基化酶基因，经DNA测序验证。接下来，使用Chromas软件进行序列比对，并采用 Kirby-Bauer纸片扩散法进行药敏试验。在所有20株多重耐药鲍曼不动杆菌中均检测到编码氨基糖苷类修饰酶的基因。aac(3')-I、aac(6')-Ib、ant(3'')-I和aph(3')-I的阳性率分别为90.0%、90.0%、85.0%和35.0%。然而，在20株多重耐药鲍曼不动杆菌中未检测到编码16S rRNA甲基化酶的基因呈阳性。多重耐药鲍曼不动杆菌的耐药性可能与氨基糖苷类修饰酶基因有关，而与16S rRNA甲基化酶基因无关。
