Kim Minsik, Heu Sunggi, Ryu Sangryeol
Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-921, South Korea.
Arch Virol. 2014 Nov;159(11):3137-40. doi: 10.1007/s00705-014-2140-1. Epub 2014 Jun 18.
A novel virulent enterobacteria phage, 4MG, which was isolated from soil near a sewer, belongs to the family Myoviridae, as it possesses an isometric head and a long contractile tail. The complete genome of 4MG consists of a double-stranded DNA with a length of 148,567 bp, a G + C content of 46.3 %, 271 open reading frames (ORFs), and 21 tRNAs. Bioinformatic analysis revealed that 4MG highly resembles "rV5-like viruses" but can be separated, together with Salmonella phage PVP-SE1 and Cronobacter sakazakii phage vB_CsaM_GAP31, as part of the subgroup "PVP-SE1-like phage".
一种从下水道附近土壤中分离出的新型烈性肠道杆菌噬菌体4MG,属于肌尾噬菌体科,因为它具有等轴状头部和长收缩尾。4MG的完整基因组由一条长度为148,567 bp的双链DNA组成,G + C含量为46.3%,有271个开放阅读框(ORF)和21个tRNA。生物信息学分析表明,4MG与“rV5样病毒”高度相似,但可与沙门氏菌噬菌体PVP-SE1和阪崎肠杆菌噬菌体vB_CsaM_GAP31一起,作为“PVP-SE1样噬菌体”亚组的一部分被区分开来。