He Ya, Wang Zheng-Huan, Wang Xiao-Ming
School of Life Sciences, East China Normal University, Shanghai 200062, China;Shanghai Science & Technology Museum, 2000 Century Avenue, Shanghai 200127, China.
School of Life Sciences, East China Normal University, Shanghai 200062, China;
Dongwuxue Yanjiu. 2014 Nov 18;35(6):528-36. doi: 10.13918/j.issn.2095-8137.2014.6.528.
Cervus sichuanicus is a species of sika deer (Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To provide scientific evidence to assist in the protection of this species, we collected Sichuan sika deer fecal samples from the Sichuan Tiebu Nature Reserve (TNR) and extracted DNA from those samples. Microsatellite loci of bovine were used for PCR amplification. After GeneScan, the genotype data were used to analyze the genetic diversity and population structure of the Sichuan sika deer in TNR. Results showed that the average expected heterozygosity of the Sichuan sika deer population in TNR was 0.562, equivalent to the average expected heterozygosity of endangered animals, such as Procapra przewalskii. Furthermore, 8 of 9 microsatellite loci significantly deviated from the Hardy-Weinberg equilibrium and two groups existed within the Sichuan sika deer TNR population. This genetic structure may be caused by a group of Manchurian sika deer (Cervus hortulorum) released in TNR.
四川梅花鹿是梅花鹿（梅花鹿组）的一个物种。迄今为止，研究主要集中在数量调查和行为研究上，目前该物种的遗传信息不足。为提供科学依据以协助保护该物种，我们从四川铁布自然保护区（TNR）采集了四川梅花鹿粪便样本，并从这些样本中提取了DNA。使用牛的微卫星位点进行PCR扩增。经过基因扫描后，利用基因型数据对TNR中四川梅花鹿的遗传多样性和种群结构进行分析。结果表明，TNR中四川梅花鹿种群的平均期望杂合度为0.562，与普氏原羚等濒危动物的平均期望杂合度相当。此外，9个微卫星位点中有8个显著偏离哈迪-温伯格平衡，且TNR的四川梅花鹿种群内存在两个群体。这种遗传结构可能是由TNR中放归的一群东北梅花鹿（Cervus hortulorum）导致的。
