Liptrott Neill J, Kendall Emily, Nieves Daniel J, Farrell John, Rannard Steve, Fernig David G, Owen Andrew
Department of Molecular & Clinical Pharmacology, University of Liverpool, UK.
Nanomedicine (Lond). 2014 Nov;9(16):2467-79. doi: 10.2217/nnm.14.38.
To investigate interactions of gold nanoparticles with primary human lymphocytes and determine if the addition of a self-assembled monolayer of 'mixed-matrix' ligands influenced these interactions.
MATERIALS & METHODS: The effect of gold nanoparticles was measured by exposure to peripheral blood mononuclear cells (PBMCs) from healthy volunteers with subsequent examination of cell proliferation, cytokine secretion and CD4(+) T-cell activation relative to controls.
Capped and as-synthesized gold nanoparticles augmented PBMC proliferation in response to phytohemagglutinin and this effect was greater for as-synthesized than for capped gold nanoparticles. Release of IL-10 and IFN-γ from PBMCs was increased and the effect was again more marked for as-synthesized than capped gold nanoparticles.
This method provides an ex vivo approach for studying the interaction of nanoparticles with the human immune system. Further research is required to determine the specific mechanisms for reduction of immune activation seen here which could then be used to design a truly 'stealth' nanoparticle.
研究金纳米颗粒与原代人淋巴细胞的相互作用，并确定添加“混合基质”配体的自组装单分子层是否会影响这些相互作用。
通过将金纳米颗粒暴露于健康志愿者的外周血单核细胞（PBMC）来测量其效果，随后相对于对照组检测细胞增殖、细胞因子分泌和CD4(+) T细胞活化情况。
包封的和刚合成的金纳米颗粒均增强了PBMC对植物血凝素的增殖反应，且刚合成的金纳米颗粒的这种效果比包封的金纳米颗粒更明显。PBMC释放的IL-10和IFN-γ增加，同样，刚合成的金纳米颗粒的效果比包封的金纳米颗粒更显著。
该方法为研究纳米颗粒与人类免疫系统的相互作用提供了一种体外研究方法。需要进一步研究以确定此处观察到的免疫激活降低的具体机制，进而可用于设计真正的“隐形”纳米颗粒。
