Department of Chemistry, Faculty of Sciences, Atatürk University , Erzurum , Turkey .
J Enzyme Inhib Med Chem. 2015 Apr;30(2):264-9. doi: 10.3109/14756366.2014.915395. Epub 2014 Jun 18.
In the present study, in order to evaluate antioxidant and radical scavenging properties of Pistachio gum (P-Gum), different bioanalytical methods such as DPPH(•) scavenging activity, DMPD(•+) radical scavenging activity, total antioxidant activity determination by ferric thiocyanate, reducing ability Fe(3+)-Fe(2+) transformation, Cuprac and FRAP assays, O2(•-) scavenging by riboflavin-methionine-illuminate system and ferrous ions (Fe(2+)) chelating activities by 2,2'-bipyridyl reagent were performed separately. P-Gum inhibited 54.2% linoleic acid peroxidation at 10 µg/ml concentration. On the other hand, BHA, BHT, α-tocopherol and trolox, pure antioxidant compounds, indicated inhibition of 80.3%, 73.5%, 36.2% and 72.0% on peroxidation of linoleic acid emulsion at the same concentration, respectively. In addition, all of sample had an effective DPPH(•), DMPD(•+) and O2(•-) scavenging, Fe(3+) reducing power by Fe(3+)-Fe(2+) transformation and FRAP assay, Cu(2+) reducing ability by Cuprac method and Fe(2+) chelating activities.
在本研究中，为了评估开心果果胶（P-Gum）的抗氧化和自由基清除特性，分别采用了不同的生物分析方法，如 DPPH（•）清除活性、DMPD（•+）自由基清除活性、铁氰化铁法测定总抗氧化活性、还原能力 Fe（3+）-Fe（2+）转化、Cuprac 和 FRAP 测定法、核黄素-蛋氨酸-光照系统清除 O2（•-）和 2,2'-联吡啶试剂螯合亚铁离子（Fe（2+））的活性。P-Gum 在 10μg/ml 浓度下抑制了 54.2%的亚油酸过氧化。另一方面，BHA、BHT、α-生育酚和 Trolox 等纯抗氧化化合物在相同浓度下对亚油酸乳液的过氧化抑制率分别为 80.3%、73.5%、36.2%和 72.0%。此外，所有样品均具有有效的 DPPH（•）、DMPD（•+）和 O2（•-）清除、Fe（3+）-Fe（2+）转化的 Fe（3+）还原能力和 FRAP 测定法、Cuprac 法的 Cu（2+）还原能力和 Fe（2+）螯合活性。
