Feng Xiao-Bo, Ke Jian-Juan, Rao Yan, Zhang Zong-Ze, Wang Yan-Lin
Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
J Huazhong Univ Sci Technolog Med Sci. 2014 Jun;34(3):403-407. doi: 10.1007/s11596-014-1291-3. Epub 2014 Jun 18.
The effect of the complement C1q expression on total hepatic ischemia-reperfusion (I/R) injury in rats was investigated. Sixty healthy male Sprague Dawley (SD) rats weighing 180-200 g were randomly divided into 5 groups: sham-operation group (S group, n=12); group of I/R for 1 h (I/R 1 h group, n=12); group of I/R for 3 h (I/R 3 h group, n=12); group of I/R for 6 h (I/R 6 h group, n=12); group of I/R for 24 h (I/R 24 h group, n=12). The hepatic I/R model of rats was established, and liver tissues were obtained 1 h, 3 h, 6 h and 24 h after hepatic I/R, respectively. Furthermore, the tissues were stained using hematoxylin-eosin, and the liver injuries of rats were observed using a microscope. The malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in liver tissue were determined. Real-time polymerase chain reaction (PCR) and Western blotting were used to detect the expression levels of C1q mRNA and protein, respectively. As compared with the S group, the histopathological changes in I/R 1 h-24 h groups were gradually aggravated with the extension of I/R time. As compared with the S group, SOD activity and MDA content in the I/R groups were reduced and increased respectively with the extension of I/R time (P<0.01). Furthermore, the C1q expression at mRNA and protein levels in the I/R groups (especially in the I/R 3 h group) was significantly higher than that in the S group (P<0.05). It is suggested that C1q expression may play a principal role in hepatic I/R injury, particularly at the early stage of perfusion.
研究补体C1q表达对大鼠全肝缺血再灌注（I/R）损伤的影响。将60只体重180 - 200 g的健康雄性Sprague Dawley（SD）大鼠随机分为5组：假手术组（S组，n = 12）；缺血再灌注1小时组（I/R 1小时组，n = 12）；缺血再灌注3小时组（I/R 3小时组，n = 12）；缺血再灌注6小时组（I/R 6小时组，n = 12）；缺血再灌注24小时组（I/R 24小时组，n = 12）。建立大鼠肝脏I/R模型，分别在肝脏I/R后1小时、3小时、6小时和24小时获取肝组织。此外，用苏木精 - 伊红对组织进行染色，并用显微镜观察大鼠的肝损伤情况。测定肝组织中丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性。分别采用实时聚合酶链反应（PCR）和蛋白质免疫印迹法检测C1q mRNA和蛋白的表达水平。与S组相比，I/R 1小时至24小时组的组织病理学变化随I/R时间延长逐渐加重。与S组相比，I/R组的SOD活性随I/R时间延长而降低，MDA含量随I/R时间延长而升高（P < 0.01）。此外，I/R组（尤其是I/R 3小时组）C1q mRNA和蛋白水平的表达明显高于S组（P < 0.05）。提示C1q表达可能在肝脏I/R损伤中起主要作用，尤其是在灌注早期。
