Pérez Andy J, Simonet Ana M, Calle Juan M, Pecio Łukasz, Guerra José O, Stochmal Anna, Macías Francisco A
Grupo de Alelopatía, Departamento de Química Orgánica, Instituto de Biomoleculas (INBIO), Facultad de Ciencias, Universidad de Cádiz, C/República Saharaui s/n, Apdo. 40, 11510-Puerto Real (Cádiz), Spain; Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, ul. Czartoryskich 8, 24-100 Puławy, Poland.
Grupo de Alelopatía, Departamento de Química Orgánica, Instituto de Biomoleculas (INBIO), Facultad de Ciencias, Universidad de Cádiz, C/República Saharaui s/n, Apdo. 40, 11510-Puerto Real (Cádiz), Spain.
Phytochemistry. 2014 Sep;105:92-100. doi: 10.1016/j.phytochem.2014.05.014. Epub 2014 Jun 14.
A bioassay-guided fractionation of Agave offoyana leaves led to the isolation of five steroidal saponins (1-5) along with six known saponins (6-11). The compounds were identified as (25R)-spirost-5-en-2α,3β-diol-12-one 3-O-{α-l-rhamnopyranosyl-(1→3)-O-β-d-glucopyranosyl-(1→2)-O-[β-d-xylopyranosyl-(1→3)]-O-β-d-glucopyranosyl-(1→4)-O-β-d-galactopyranoside} (1), (25R)-spirost-5-en-3β-ol-12-one 3-O-{α-l-rhamnopyranosyl-(1→3)-O-β-d-glucopyranosyl-(1→2)-O-[β-d-xylopyranosyl-(1→3)]-O-β-d-glucopyranosyl-(1→4)-O-β-d-galactopyranoside} (2), (25R)-spirost-5-en-3β-ol-12-one 3-O-{β-d-xylopyranosyl-(1→3)-O-β-d-glucopyranosyl-(1→2)-O-[β-d-xylopyranosyl-(1→3)]-O-β-d-glucopyranosyl-(1→4)-O-β-d-galactopyranoside} (3), (25R)-26-O-β-d-glucopyranosylfurost-5-en-3β,22α,26-triol-12-one 3-O-{α-l-rhamnopyranosyl-(1→3)-O-β-d-glucopyranosyl-(1→2)-O-[β-d-xylopyranosyl-(1→3)]-O-β-d-glucopyranosyl-(1→4)-O-β-d-galactopyranoside} (4) and (25R)-26-O-β-d-glucopyranosylfurost-5-en-3β,22α,26-triol-12-one 3-O-{β-d-xylopyranosyl-(1→3)-O-β-d-glucopyranosyl-(1→2)-O-[β-d-xylopyranosyl-(1→3)]-O-β-d-glucopyranosyl-(1→4)-O-β-d-galactopyranoside} (5) by comprehensive spectroscopic analysis, including one- and two-dimensional NMR techniques, mass spectrometry and chemical methods. The phytotoxicity of the isolated compounds on the standard target species Lactuca sativa was evaluated.
对龙舌兰（Agave offoyana）叶片进行生物测定导向的分级分离，得到了5种甾体皂苷（1 - 5）以及6种已知皂苷（6 - 11）。通过综合光谱分析，包括一维和二维核磁共振技术、质谱和化学方法，将这些化合物鉴定为（25R）-螺甾-5-烯-2α，3β-二醇-12-酮3 - O - {α-L-鼠李糖基-（1→3）-O-β-D-葡萄糖基-（1→2）-O-[β-D-木糖基-（1→3）]-O-β-D-葡萄糖基-（1→4）-O-β-D-半乳糖苷}（1）、（25R）-螺甾-5-烯-3β-醇-12-酮3 - O - {α-L-鼠李糖基-（1→3）-O-β-D-葡萄糖基-（1→2）-O-[β-D-木糖基-（1→3）]-O-β-D-葡萄糖基-（1→4）-O-β-D-半乳糖苷}（2）、（25R）-螺甾-5-烯-3β-醇-12-酮3 - O - {β-D-木糖基-（1→3）-O-β-D-葡萄糖基-（1→2）-O-[β-D-木糖基-（1→3）]-O-β-D-葡萄糖基-（1→4）-O-β-D-半乳糖苷}（3）、（25R）-26 - O-β-D-葡萄糖基呋甾-5-烯-3β，22α，26-三醇-12-酮3 - O - {α-L-鼠李糖基-（1→3）-O-β-D-葡萄糖基-（1→2）-O-[β-D-木糖基-（1→3）]-O-β-D-葡萄糖基-（1→4）-O-β-D-半乳糖苷}（4）和（25R）-26 - O-β-D-葡萄糖基呋甾-5-烯-3β，22α，26-三醇-12-酮3 - O - {β-D-木糖基-（1→3）-O-β-D-葡萄糖基-（1→2）-O-[β-D-木糖基-（1→3）]-O-β-D-葡萄糖基-（1→4）-O-β-D-半乳糖苷}（5）。评估了分离得到的化合物对标准靶标物种莴苣（Lactuca sativa）的植物毒性。
