Santos-Zea Liliana, Fajardo-Ramírez Oscar R, Romo-López Irasema, Gutiérrez-Uribe Janet A
Centro de Biotecnología-FEMSA, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Campus Monterrey, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, NL, Mexico.
Centro de Innovación y Transferencia en Salud, Tecnológico de Monterrey, Campus Monterrey, Av. Morones Prieto 3000 Pte., C.P. 64710, Monterrey, NL, Mexico.
Plant Foods Hum Nutr. 2016 Mar;71(1):57-63. doi: 10.1007/s11130-015-0525-2.
Separation of potentially bioactive components from foods and plant extracts is one of the main challenges for their study. Centrifugal partition chromatography has been a successful technique for the screening and identification of molecules with bioactive potential, such as steroidal saponins. Agave is a source of steroidal saponins with anticancer potential, though the activity of these compounds in concentrated agave sap has not been yet explored. In this study, fast centrifugal partition chromatography (FCPC) was used coupled with in vitro tests on HT-29 cells as a screening procedure to identify apoptotic saponins from an acetonic extract of concentrated agave sap. The three most bioactive fractions obtained by FCPC at partition coefficients between 0.23 and 0.4 contained steroidal saponins, predominantly magueyoside b. Flow cytometry analysis determined that the fraction rich in kammogenin and manogenin glycosides induced apoptosis, but when gentrogenin and hecogenin glycosides were also found in the fraction, a necrotic effect was observed. In conclusion, this study provides the evidence that steroidal saponins in concentrated agave sap were potential inductors of apoptosis and that it was possible to separate them using fast centrifugal partition chromatography.
从食品和植物提取物中分离具有潜在生物活性的成分是对其进行研究的主要挑战之一。离心分配色谱法是一种成功的技术，可用于筛选和鉴定具有生物活性潜力的分子，如甾体皂苷。龙舌兰是甾体皂苷的来源，具有抗癌潜力，不过这些化合物在浓缩龙舌兰汁中的活性尚未得到探索。在本研究中，快速离心分配色谱法（FCPC）与对HT-29细胞的体外试验相结合，作为一种筛选程序，以从浓缩龙舌兰汁的丙酮提取物中鉴定凋亡皂苷。通过FCPC在分配系数为0.23至0.4之间获得的三种生物活性最强的馏分含有甾体皂苷，主要是龙舌兰皂苷b。流式细胞术分析确定，富含卡茂皂苷元和马尼皂苷元糖苷的馏分诱导细胞凋亡，但当该馏分中也发现龙胆皂苷元和海柯皂苷元糖苷时，则观察到坏死效应。总之，本研究提供了证据，表明浓缩龙舌兰汁中的甾体皂苷是细胞凋亡的潜在诱导剂，并且可以使用快速离心分配色谱法将它们分离。
