Department of Dermatology, No,1 Hospital of China Medical University, Shenyang 110001, PR China.
Virol J. 2010 Nov 4;7:301. doi: 10.1186/1743-422X-7-301.
Local hyperthermia has been successfully used in the treatment of viral warts. However, the mechanism of action has largely remained unclear. CCL-20 (also known as MIP-3α) is the most potent chemokine for recruitment of Langerhans cell (LC) precursors into the skin. CCL-20 expression can be increased by TNF-α and IL-1α. The effects of local hyperthermia on the mRNA expressions of CCL-20, TNF-α, IL-1α have been investigated in both condyloma acuminata (CA) and normal skin. Under an organotypic culture condition, fresh CA and normal skin were subjected to surface heating at 37°C, 42°C and 45°C for 30 mins, respectively.
The mRNA expressions of CCL-20 and IL-1α in CA specimen were significantly higher than those in normal skin. Local hyperthermia at 42°C and 45°C significantly decreased the mRNA levels of CCL-20 and IL-1α, as compared with the control groups (p < 0.01). The decrease of CCL-20 was well correlated with that of IL-1α. The expression of TNF-α in CA remained unchanged in spite of the temperature variation. Local hyperthermia at 45°C concomitantly increased the mRNA expression of CCL-20 and IL-1α in normal skin.
Our study suggests that hyperthermia decreases the expression of CCL-20 with concomitant decrease in IL-1α, and reduce the number of Langerhans cells in HPV infected skin.
局部热疗已成功应用于治疗病毒性疣。然而，其作用机制在很大程度上仍不清楚。CCL-20（也称为 MIP-3α）是招募朗格汉斯细胞（LC）前体细胞进入皮肤的最有效趋化因子。CCL-20 的表达可被 TNF-α 和 IL-1α 上调。本研究探讨了局部热疗对尖锐湿疣（CA）和正常皮肤中 CCL-20、TNF-α、IL-1α mRNA 表达的影响。在器官型培养条件下，新鲜 CA 和正常皮肤分别在 37°C、42°C 和 45°C 下表面加热 30 分钟。
CA 标本中 CCL-20 和 IL-1α 的 mRNA 表达明显高于正常皮肤。与对照组相比，42°C 和 45°C 局部热疗显著降低了 CCL-20 和 IL-1α 的 mRNA 水平（p < 0.01）。CCL-20 的减少与 IL-1α 的减少呈良好相关性。CA 中 TNF-α 的表达尽管温度变化仍保持不变。45°C 局部热疗同时增加了正常皮肤中 CCL-20 和 IL-1α 的 mRNA 表达。
本研究表明，热疗可降低 HPV 感染皮肤中 CCL-20 的表达，同时降低 IL-1α 的表达，减少朗格汉斯细胞的数量。
