Clausen Anders R, Mutahir Zeeshan, Munch-Petersen Birgitte, Piškur Jure
a Department of Biology , Lund University , Sweden.
Nucleosides Nucleotides Nucleic Acids. 2014;33(4-6):291-5. doi: 10.1080/15257770.2013.853782.
Deoxyribonucleoside kinases phosphorylate deoxyribonucleosides into the corresponding 5'-monophosphate deoxyribonucleosides to supply the cell with nucleic acid precursors. In mitochondrial fractions of the model plant Arabidopsis thaliana, we detected deoxyadenosine and thymidine kinase activities, while the cytosol fraction contained six-fold lower activity and chloroplasts contained no measurable activities. In addition, a mitochondrial fraction isolated from the potato Solanum tuberosum contained thymidine kinase and deoxyadenosine kinase activities. We conclude that an active salvage of deoxyribonucleosides in plants takes place in their mitochondria. In general, the observed localization of the plant dNK activities in the mitochondrion suggests that plants have a different organization of the deoxyribonucleoside salvage compared to mammals.
脱氧核糖核苷激酶将脱氧核糖核苷磷酸化为相应的5'-单磷酸脱氧核糖核苷，为细胞提供核酸前体。在模式植物拟南芥的线粒体部分，我们检测到脱氧腺苷和胸苷激酶活性，而胞质溶胶部分的活性低六倍，叶绿体中没有可测量的活性。此外，从马铃薯中分离出的线粒体部分含有胸苷激酶和脱氧腺苷激酶活性。我们得出结论，植物中脱氧核糖核苷的活性补救发生在线粒体中。一般来说，观察到的植物dNK活性在线粒体中的定位表明，与哺乳动物相比，植物的脱氧核糖核苷补救组织不同。
