Hattori Yorito, Kitamura Akihiro, Nagatsuka Kazuyuki, Ihara Masafumi
Department of Regenerative Medicine and Tissue Engineering, National Cerebral and Cardiovascular Center, Suita, Osaka, Japan; Department of Neurology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Department of Neurology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
PLoS One. 2014 Jun 18;9(6):e100257. doi: 10.1371/journal.pone.0100257. eCollection 2014.
Carotid artery occlusive disease gradually develops over time, eventually leading to cerebral infarction and high mortality rate. Animal models replicating cerebral infarction resulting from carotid artery occlusive disease have thus been developed to test potential novel treatments, which could be subsequently administered clinically.
Adult C57BL/6J male mice were subjected to ameroid constrictor (AC) placement to gradually narrow the bilateral common carotid arteries. Cerebral blood flow (CBF) was measured at several time points. At 7 and 28 days post-operation, post-mortem brain samples were analyzed for ischemic changes.
The mortality rate was 58.8% at 28 days post-operation. Surviving mice with AC showed continuous reduction of CBF by up to 70% of the baseline level at 28 days. Most of the mice (75%) showed multiple cerebral infarctions in the gray and white matter. Non-surviving mice showed critical CBF reduction below 20-30% of the baseline level before death.
The application of the AC on the bilateral common carotid arteries in mice could offer a reliable model of severe cerebrovascular insufficiency due to carotid artery occlusive disease and may thus be useful in exploring pharmacological intervention in stroke through monitoring survival rate, infarct formation, and CBF profile.
颈动脉闭塞性疾病会随着时间逐渐发展，最终导致脑梗死且死亡率很高。因此，已开发出复制由颈动脉闭塞性疾病导致脑梗死的动物模型，以测试潜在的新型治疗方法，这些方法随后可应用于临床。
对成年C57BL/6J雄性小鼠放置阿梅里德缩窄器（AC）以逐渐使双侧颈总动脉变窄。在几个时间点测量脑血流量（CBF）。在术后7天和28天，对死后的脑样本进行缺血变化分析。
术后28天死亡率为58.8%。存活的放置AC的小鼠在28天时CBF持续降低，降至基线水平的70%。大多数小鼠（75%）在灰质和白质中出现多处脑梗死。未存活的小鼠在死亡前CBF严重降低至基线水平的20%-30%以下。
在小鼠双侧颈总动脉上应用AC可提供一种因颈动脉闭塞性疾病导致严重脑血管功能不全的可靠模型，因此可能有助于通过监测存活率、梗死形成和CBF情况来探索中风的药物干预。
