Ning Xianbin, Luo Qi, Li Chuang, Ding Zhaoyi, Pang Jinfeng, Zhao Changfu
Department of Neurosurgery, The First Affiliated Hospital of Jilin University, Changchun, Department of Neurosurgery, The Affiliated Hospital of Beihua University, Jilin, China.
Department of Neurosurgery, The First Affiliated Hospital of Jilin University, Changchun, China.
Int J Med Mushrooms. 2014;16(1):29-36. doi: 10.1615/intjmedmushr.v16.i1.30.
This study aimed to investigate the inhibitory roles of a polysaccharide extract from Inonotus obliquus on U251 human neurogliocytoma cells cultured in vitro. After administering the polysaccharide extract from I. obliquus to U251 cells cultivated in vitro, methyl thiazolyl tetrazoliym assay was performed to measure the inhibitory effects of the extract on tumor cell proliferation. The expression of the apoptosis-related proteins Bcl-2 and caspase-3 were determined by Western blotting. Different concentrations of I. obliquus extract (25, 50, 100, 200, and 500 µg/mL) were added to U251 cells at 24, 48, and 72 hours. Methyl thiazolyl tetrazoliym assay showed that the inhibition ratio increased with increased extract concentration and prolonged treatment duration. The I. obliquus extract sharply decreased the expression of Bcl-2 but dramatically increased the expression of caspase-3. This function was gradually enhanced with increased drug concentration and prolonged treatment duration. The I. obliquus extract can inhibit the proliferation of tumor cells. This inhibition function is closely related to the downregulation of Bcl-2 and the upregulation of caspase-3.
本研究旨在探讨桦褐孔菌多糖提取物对体外培养的U251人神经胶质瘤细胞的抑制作用。将桦褐孔菌多糖提取物作用于体外培养的U251细胞后，采用甲基噻唑基四氮唑蓝法检测提取物对肿瘤细胞增殖的抑制作用。通过蛋白质免疫印迹法检测凋亡相关蛋白Bcl-2和半胱天冬酶-3的表达。在24、48和72小时时，将不同浓度（25、50、100、200和500µg/mL）的桦褐孔菌提取物加入U251细胞中。甲基噻唑基四氮唑蓝法检测结果显示，随着提取物浓度的增加和处理时间的延长，抑制率升高。桦褐孔菌提取物可使Bcl-2表达急剧下降，但使半胱天冬酶-3表达显著增加。该作用随药物浓度的增加和处理时间的延长而逐渐增强。桦褐孔菌提取物可抑制肿瘤细胞增殖。这种抑制作用与Bcl-2的下调和半胱天冬酶-3的上调密切相关。
