Li Jiawei, Qu Chao, Li Fangfang, Chen Yifang, Zheng Jinjuan, Xiao Yao, Jin Quanxin, Jin Guihua, Huang Xuezhu, Jin Dan
Immunology and Pathogenic Biology Key Laboratory of Jilin Province, Yanbian University, Yanji, China.
Department of Anesthesiology, Affiliated Hospital of Yanbian University, Yanji, China.
Front Pharmacol. 2021 Feb 2;11:621835. doi: 10.3389/fphar.2020.621835. eCollection 2020.
polysaccharide (IOP), the primary constituent of the parasitic fungus , has anti-tumor, anti-inflammatory, anti-oxidation effects. However, the roles of IOP on colitis-associated cancer (CAC) are still unclear. Herein, we tested the efficacy of IOP using a mouse model of CAC induced by azoxymethane and dextran sulfate sodium (AOM/DSS). We confirmed that intragastric administration of IOP decreased CAC-induced body weight loss, colon tissue damage, colon shortening, and expression of proinflammatory mediators. Meanwhile, IOP treatment increased in expression of the NLRP3 inflammasome, IL-1β, and IL-18 in the colon of CAC mice. Moreover, , IOP inhibited the proliferation of SW620 colorectal cancer cells. Finally, overexpression of NLRP3 with plasmid transfection could further enhance the activation of NLRP3 inflammasome by IOP. Taken together, these results suggest that IOP suppresses the development of CAC, possibly by activation of the NLRP3 inflammasome, and reveal that IOP may be a therapeutic drug candidate for CAC.
多聚多糖（IOP）是寄生真菌的主要成分，具有抗肿瘤、抗炎、抗氧化作用。然而，IOP在结肠炎相关癌症（CAC）中的作用仍不清楚。在此，我们使用由氧化偶氮甲烷和葡聚糖硫酸钠（AOM/DSS）诱导的CAC小鼠模型测试了IOP的功效。我们证实，胃内给予IOP可减轻CAC诱导的体重减轻、结肠组织损伤、结肠缩短以及促炎介质的表达。同时，IOP治疗增加了CAC小鼠结肠中NLRP3炎性小体、IL-1β和IL-18的表达。此外，IOP抑制SW620结肠癌细胞的增殖。最后，用质粒转染过表达NLRP3可进一步增强IOP对NLRP3炎性小体的激活。综上所述，这些结果表明IOP可能通过激活NLRP3炎性小体抑制CAC的发展，并揭示IOP可能是CAC的一种治疗候选药物。
