Gaston Joel, Bartlett Rebecca S, Klemuk Sarah A, Thibeault Susan L
Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Ann Otol Rhinol Laryngol. 2014 Dec;123(12):866-74. doi: 10.1177/0003489414539131. Epub 2014 Jun 18.
Biomaterials able to mimic the mechanical properties of vocal fold tissue may be particularly useful for furnishing a 3-dimensional microenvironment allowing for in vitro investigation of cell and molecular responses to vibration. Motivated by the dearth of biomaterials available for use in an in vitro model for vocal fold tissue, we investigated polyether polyurethane (PEU) matrices, which are porous, mechanically tunable biomaterials that are inexpensive and require only standard laboratory equipment for fabrication.
Rheology, dynamic mechanical analysis, and scanning electron microscopy were performed on PEU matrices at 5%, 10%, and 20% w/v mass concentrations.
For 5%, 10%, and 20% w/v concentrations, shear storage moduli were 2 kPa, 3.4 kPa, and 6 kPa, respectively, with shear loss moduli being 0.2 kPa, 0.38 kPa, and 0.62 kPa, respectively. Storage moduli responded to applied frequency as a linear function. Mercury intrusion porosimetry revealed that all 3 mass concentrations of PEU have a similar overall percentage porosity but differ in pore architecture.
Twenty-µm diameter pores are ideal for cell seeding, and a range of mechanical properties indicates that the lower [corrected] mass concentration PEU formulations are best suited for mimicking the viscoelastic properties of vocal fold tissue for in vitro research.
能够模拟声带组织力学特性的生物材料可能对构建三维微环境特别有用，该微环境可用于体外研究细胞和分子对振动的反应。鉴于用于声带组织体外模型的生物材料匮乏，我们研究了聚醚聚氨酯（PEU）基质，它是一种多孔、力学性能可调节的生物材料，价格低廉，制造仅需标准实验室设备。
对质量浓度为5%、10%和20%的PEU基质进行流变学、动态力学分析和扫描电子显微镜检查。
对于5%、10%和20%的质量浓度，剪切储能模量分别为2 kPa、3.4 kPa和6 kPa，剪切损耗模量分别为0.2 kPa、0.38 kPa和0.62 kPa。储能模量随施加频率呈线性函数响应。压汞孔隙率测定显示，所有3种质量浓度的PEU总体孔隙率相似，但孔隙结构不同。
直径20 µm的孔隙最适合细胞接种，一系列力学性能表明，较低质量浓度的PEU配方最适合在体外研究中模拟声带组织的粘弹性特性。
