Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, PR China.
Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):3104-3108. doi: 10.1099/ijs.0.066373-0. Epub 2014 Jun 18.
Two Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated BM5-7(T) and BM9-1(T) were isolated from soil of the root system of a mangrove forest. Phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates belong to the genus Martelella. The chemotaxonomic characteristics of these isolates included the presence of C19 : 0 cyclo ω8c and C18 : 1ω7c as the major cellular fatty acids and Q-10 as the dominant ubiquinone. The genomic DNA G+C contents of strains BM5-7(T) and BM9-1(T) were 61.0 and 59.7 mol% (HPLC method), respectively. The 16S rRNA gene sequence similarity between the two strains was 98.1 %, but DNA-DNA hybridization indicated 44 % relatedness. Strains BM5-7(T) and BM9-1(T) exhibited 16S rRNA gene sequence similarities of 98.0-99.2 % and 97.7-98.1 %, respectively, with type strains of Martelella endophytica and Martelella mediterranea. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strains BM5-7(T) and BM9-1(T) are representatives of two novel species of the genus Martelella, for which the names Martelella radicis sp. nov. (type strain BM5-7(T) = DSM 28101(T) = LMG 27958(T)) and Martelella mangrovi sp. nov. (type strain BM9-1(T) = DSM 28102(T) = LMG 27959(T)) are proposed.
两株革兰氏阴性、非运动、杆状细菌菌株，分别命名为 BM5-7(T) 和 BM9-1(T)，从红树林根系土壤中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，这两个分离物属于 Martelella 属。这些分离物的化学分类特征包括存在 C19 : 0 环 ω8c 和 C18 : 1ω7c 作为主要的细胞脂肪酸和 Q-10 作为主要的泛醌。菌株 BM5-7(T) 和 BM9-1(T) 的基因组 DNA G+C 含量分别为 61.0 和 59.7 mol%（HPLC 法）。两株菌的 16S rRNA 基因序列相似度为 98.1%，但 DNA-DNA 杂交表明它们的相关性为 44%。菌株 BM5-7(T) 和 BM9-1(T) 与 Martelella endophytica 和 Martelella mediterranea 的 16S rRNA 基因序列相似度分别为 98.0-99.2%和 97.7-98.1%。综合表型、系统发育和 DNA-DNA 同源性研究的数据表明，菌株 BM5-7(T) 和 BM9-1(T) 是 Martelella 属的两个新种的代表，分别命名为 Martelella radicis sp. nov.（模式菌株 BM5-7(T) = DSM 28101(T) = LMG 27958(T)）和 Martelella mangrovi sp. nov.（模式菌株 BM9-1(T) = DSM 28102(T) = LMG 27959(T)）。
