Institute of Marine Drugs and School of Pharmaceutical Sciences, Guangxi University of Chinese Medicine, NO. 13 Wuhe Road, Nanning, 530200, People's Republic of China.
Arch Microbiol. 2021 May;203(4):1779-1786. doi: 10.1007/s00203-020-02178-2. Epub 2021 Jan 20.
Strain BGMRC 2036 was isolated from rhizosphere soil of Bruguiear gymnorrhiza collected from the Beibu Gulf of China. Optimum growth occurred at 28 °C, pH 7.0, and under the conditions of 3-5% (w/v) NaCl. The phylogenetic comparisons of 16S rRNA gene sequences displayed that strain BGMRC 2036 was closely related to Martelella limonii NBRC109441 (96.6% sequence similarity), M. mediterranea CGMCC 1.12224 (96.5%), M. lutilitoris GH2-6 (96.5%), M. radicis BM5-7 (96.2%), and M. mangrove BM9-1 (95.9%), M. suaedae NBRC109440 (95.8%). The phylogenomic tree based on the up-to-date bacterial core gene set indicated that the strain BGMRC 2036 form a clade formed with members of the genera Martelella. The major polar lipids include phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphotidylinositol, two unidentified phospholipids, and three unidentified ninhydrin positive phospholipids. The major respiratory quinone is Q-10, which is similar to those of genera Martelella. The main cellular fatty acids are C ω7c, C, and C aldehyde. Genome sequencing revealed a genome size of 4.99 Mbp and a G + C content of 62.3 mol%. Pairwise comparison of the genomes of the new strain BGMRC 2036 and the three reference strains M. endophytica YC 6887, M. mediterranea CGMCC 1.12224, and M. mangrovi USBA-857 indicated that gANI value was lower than 81% and a digital DNA-DNA hybridization value was lower than 27%. The strain BGMRC 2036 possessed genes putatively encoding riboflavin synthesis and flavodoxin A polyphasic taxonomic study suggested that strain BGMRC 2036 represented a novel species belonging to the genus Martelella, and it was named Martelella alba sp. nov. The type strain is BGMRC 2036 (=KCTC 52121 =NBRC 111908).
菌株 BGMRC 2036 是从采自中国北部湾的 Bruguiera gymnorrhiza 根际土壤中分离得到的。其最适生长温度为 28°C，pH 值为 7.0，在 3-5%（w/v）NaCl 条件下生长良好。16S rRNA 基因序列的系统发育比较显示，菌株 BGMRC 2036 与 Martelella limonii NBRC109441（96.6%序列相似性）、M. mediterranea CGMCC 1.12224（96.5%）、M. lutilitoris GH2-6（96.5%）、M. radicis BM5-7（96.2%）和 M. mangrove BM9-1（95.9%）、M. suaedae NBRC109440（95.8%）密切相关。基于最新细菌核心基因集的系统发育树表明，菌株 BGMRC 2036 与 Martelella 属的成员形成一个分支。主要极性脂包括磷脂酰甲乙醇胺、磷脂酰甘油、磷脂酰胆碱、磷脂酰肌醇、两种未鉴定的磷脂和三种未鉴定的茚三酮阳性磷脂。主要呼吸醌为 Q-10，与 Martelella 属的呼吸醌相似。主要细胞脂肪酸为 C ω7c、C 和 C 醛。基因组测序揭示了一个 4.99 Mbp 的基因组大小和 62.3 mol%的 G+C 含量。新菌株 BGMRC 2036 与 3 株参考菌株 M. endophytica YC 6887、M. mediterranea CGMCC 1.12224 和 M. mangrovi USBA-857 的基因组进行成对比较，表明 gANI 值低于 81%，数字 DNA-DNA 杂交值低于 27%。该菌株 BGMRC 2036 具有编码核黄素合成和黄素蛋白 A 的基因。多相分类学研究表明，菌株 BGMRC 2036 代表一个属于 Martelella 属的新种，命名为 Martelella alba sp. nov. 。模式菌株为 BGMRC 2036（=KCTC 52121=NBRC 111908）。
