Liao Hu, Li Yuqian, Zhang Mengjia, Lin Xiaolan, Lai Qiliang, Tian Yun
Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, PR China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4851-4856. doi: 10.1099/ijsem.0.002393. Epub 2017 Oct 12.
A Gram-stain-negative, facultative anaerobic, yellow-pigmented, rod-shaped, non-flagellum and non-motile bacterial strain, designed C9-11, was isolated from mangrove sediment in Zhangzhou, Fujian Province, China. Growth was observed at temperatures from 15 to 37 °C (optimum 30 °C), at salinities from 0 to 4 % (optimum 0.5-2 %) and at pH from 6 to 8 (optimum 7). Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain C9-11 was a member of the genus Altererythrobacter. Strain C9-11 was related most closely to Altererythrobacter oceanensis Y2 (96.8 % 16S rRNA gene sequence similarity), followed by Altererythrobacter marinus H32 (96.5 %). The G+C content of the genomic DNA was 63.5 mol%. The dominant fatty acids were C18 : 1ω7c (41.6 %), C17 : 1ω6c (15.4 %), 11-methyl C18 : 1ω7c (8.1 %), summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c 7.4 %) and C16 : 0 (6.2 %). The predominant respiratory quinone was ubiquinone-10 and the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. According to its morphology, physiology, fatty acid composition and 16S rRNA gene sequence analysis, strain C9-11belongs to genus Altererythrobacter, but can readily be distinguished from recognized species. Strain C9-11 is proposed as the type strain of a novel species of the genus Altererythrobacter, for which the nameAltererythrobactermangrovi sp. nov (=MCCC 1K03311=JCM 32056) is proposed.
从中国福建省漳州市的红树林沉积物中分离出一株革兰氏染色阴性、兼性厌氧、黄色色素、杆状、无鞭毛且不运动的细菌菌株，命名为C9-11。该菌株在15至37°C（最适温度30°C）、盐度0至4%（最适盐度0.5-2%）以及pH值6至8（最适pH值7）的条件下均可生长。基于16S rRNA基因序列的系统发育分析表明，菌株C9-11是交替赤杆菌属的成员。菌株C9-11与海洋交替赤杆菌Y2关系最为密切（16S rRNA基因序列相似度为96.8%），其次是海生交替赤杆菌H32（96.5%）。基因组DNA的G+C含量为63.5 mol%。主要脂肪酸为C18:1ω7c（41.6%）、C17:1ω6c（15.4%）、11-甲基C18:1ω7c（8.1%）、特征性总和3（包含C16:1ω7c和/或C16:1ω6c 7.4%）以及C16:0（6.2%）。主要呼吸醌为泛醌-10，主要极性脂为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和鞘糖脂。根据其形态、生理、脂肪酸组成和16S rRNA基因序列分析，菌株C9-11属于交替赤杆菌属，但可轻易与已知物种区分开来。菌株C9-11被提议作为交替赤杆菌属一个新物种的模式菌株，为此提议将其命名为红树林交替赤杆菌新种（Altererythrobacter mangrovi sp. nov）（=MCCC 1K03311=JCM 32056）。
