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下一代益生元促进双歧杆菌的选择性生长，抑制 。

Next-generation prebiotic promotes selective growth of bifidobacteria, suppressing .

机构信息

Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Nonoichi, Ishikawa, Japan.

Faculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan.

出版信息

Gut Microbes. 2021 Jan-Dec;13(1):1973835. doi: 10.1080/19490976.2021.1973835.

DOI:10.1080/19490976.2021.1973835
PMID:34553672
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8475593/
Abstract

Certain existing prebiotics meant to facilitate the growth of beneficial bacteria in the intestine also promote the growth of other prominent bacteria. Therefore, the growth-promoting effects of β-galactosides on intestinal bacteria were analyzed. Galactosyl-β1,4-l-rhamnose (Gal-β1,4-Rha) selectively promoted the growth of subsp. 105-A (JCM 31944) has multiple solute-binding proteins belonging to ATP-binding cassette transporters for sugars. Each strain in the library of 11 subsp. mutants, in which each gene of the solute-binding protein was disrupted, was cultured in a medium containing Gal-β1,4-Rha as the sole carbon source, and only the BL105A_0502 gene-disruption mutant showed delayed and reduced growth compared to the wild-type strain. BL105A_0502 homolog is highly conserved in bifidobacteria. In a Gal-β1,4-Rha-containing medium, subsp. JCM 1222, which possesses BLIJ_2090, a homologous protein to BL105A_0502, suppressed the growth of enteric pathogen , whereas the BLIJ_2090 gene-disrupted mutant did not. , administration of and Gal-β1,4-Rha alleviated infection-related weight loss in mice. We have successfully screened Gal-β1,4-Rha as a next-generation prebiotic candidate that specifically promotes the growth of beneficial bacteria without promoting the growth of prominent bacteria and pathogens.

摘要

某些现有的旨在促进肠道内有益细菌生长的益生元也会促进其他突出细菌的生长。因此，分析了β-半乳糖苷对肠道细菌的促生长作用。半乳糖基-β1,4-L-鼠李糖（Gal-β1,4-Rha）选择性地促进了 subsp. 105-A（JCM 31944）的生长，该菌具有多种属于 ATP 结合盒转运体的糖结合蛋白。在 11 个 subsp. 突变体文库的每个菌株中，每个溶质结合蛋白的基因都被破坏，在含有 Gal-β1,4-Rha 作为唯一碳源的培养基中进行培养，只有 BL105A_0502 基因破坏突变体的生长比野生型菌株延迟且减少。BL105A_0502 同源物在双歧杆菌中高度保守。在含有 Gal-β1,4-Rha 的培养基中，具有 BLIJ_2090 同源蛋白的 subsp. JCM 1222 抑制了肠道病原体 的生长，而 BLIJ_2090 基因破坏突变体则没有。在感染期间，和 Gal-β1,4-Rha 的给药缓解了小鼠的体重减轻。我们已经成功筛选出 Gal-β1,4-Rha 作为下一代益生元候选物，它可以特异性地促进有益细菌的生长，而不会促进突出细菌和病原体的生长。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e023/8475593/53bca5645405/KGMI_A_1973835_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e023/8475593/6e6235858323/KGMI_A_1973835_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e023/8475593/e1eab72ee891/KGMI_A_1973835_F0002_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e023/8475593/44eaecce89f2/KGMI_A_1973835_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e023/8475593/b956d0689dcf/KGMI_A_1973835_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e023/8475593/53bca5645405/KGMI_A_1973835_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e023/8475593/6e6235858323/KGMI_A_1973835_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e023/8475593/e1eab72ee891/KGMI_A_1973835_F0002_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e023/8475593/44eaecce89f2/KGMI_A_1973835_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e023/8475593/b956d0689dcf/KGMI_A_1973835_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e023/8475593/53bca5645405/KGMI_A_1973835_F0005_OC.jpg

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