Department of Anatomy and Cell Biology, Collage of Medicine, Han-Yang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 133-791, Korea.
Exercise Biochemistry Laboratory, Korea National Sport University, Seoul, 138-763, Korea.
Nutr Res Pract. 2014 Jun;8(3):284-91. doi: 10.4162/nrp.2014.8.3.284. Epub 2014 May 15.
BACKGROUND/OBJECTIVES: The aim of this study was to investigate the effects of exercise (EX) and Korean red ginseng (KRG) on inflammation mechanism in aging model rats with diet-induced atherosclerosis.
MATERIALS/METHODS: Forty-eight male Sprague-Dawley rats were divided into 6 groups: Young control (Y-C), Aging control (A-C), A-C with HFD (AHF), AHF with EX (AHF-EX), AHF-EX with KRG (AHF-EX+RG), and AHF with KRG (AHF-RG). Aging was induced by D-gal (100mg/kg) and atherosclerosis was induced by HFD (60% fat) for 9 weeks. The experimental rats were performed swimming (60 min/day, 5 days/week) and supplied KRG orally (dose of 200 mg/kg) for 8 weeks. All rat aorta samples were harvested for biochemical and immunohistochemical analyses.
The EX and KRG supplementation significantly inhibited body weight and levels of TC, TG, LDL-C, and enhance of HDL-C compared with untreated AHF groups. AHF-EX, AHF-EX+RG, and AHF-RG group showed a decreased plasma CRP and increase plasma NO activities compared to AHF group. In addition, these groups revealed reduced 4-HNE, NF-kB, TNF-α, IL-6, COX-2, ICAM-1, VCAM-1 and enhanced eNOS expression in the aorta.
These results suggest that EX alone, KRG alone, and combined treatment of EX and KRG may be an effective anti-inflammatory therapeutic for the atherosclerosis, possibly acting via the decreased of CRP and pro-inflammation proteins and the increased NO and eNOS.
背景/目的：本研究旨在探讨运动（EX）和高丽红参（KRG）对饮食诱导动脉粥样硬化的衰老模型大鼠炎症机制的影响。
材料/方法：48 只雄性 Sprague-Dawley 大鼠分为 6 组：年轻对照组（Y-C）、衰老对照组（A-C）、高脂饮食诱导衰老组（AHF）、高脂饮食诱导衰老运动组（AHF-EX）、高脂饮食诱导衰老运动加红参组（AHF-EX+RG）和高脂饮食诱导衰老加红参组（AHF-RG）。通过 D-半乳糖（100mg/kg）诱导衰老，通过高脂饮食（60%脂肪）诱导 9 周动脉粥样硬化。实验大鼠进行游泳（每天 60 分钟，每周 5 天），并口服红参（剂量 200mg/kg）8 周。采集所有大鼠主动脉样本进行生化和免疫组织化学分析。
与未处理的 AHF 组相比，EX 和 KRG 补充显著抑制了体重和 TC、TG、LDL-C 水平，并提高了 HDL-C 水平。与 AHF 组相比，AHF-EX、AHF-EX+RG 和 AHF-RG 组的血浆 CRP 降低，血浆 NO 活性增加。此外，这些组显示出主动脉中 4-HNE、NF-kB、TNF-α、IL-6、COX-2、ICAM-1、VCAM-1 减少和 eNOS 表达增加。
这些结果表明，EX 单独、KRG 单独以及 EX 和 KRG 的联合治疗可能是一种有效的抗动脉粥样硬化炎症治疗方法，可能通过降低 CRP 和促炎蛋白以及增加 NO 和 eNOS 来发挥作用。
