Catechin Isolated from Leaves Inhibit Growth through the Induction of Oxidative Stress.

BACKGROUND

Resistance of antimalarial drugs to has become a major concern in malaria eradication. Although it is also affected by several socioeconomic factors, a new antiplasmodial agent is needed for a global malaria control program.

OBJECTIVE

In this study, we attempted to uncover the antiplasmodial properties of , an Indonesian medicinal plant, along with the responsible compound and its possible mechanism.

MATERIALS AND METHODS

The leaves were ethanol extracted and fractionated based on their polarity using -hexane, ethyl acetate, and water. The antiplasmodial activity was tested against chloroquine-resistant at 100 μg/ml for 72 h. The active compound of the most active ethyl acetate fraction was subsequently isolated using column chromatography and identified by nuclear magnetic resonance.

RESULTS

The IC of (+)-catechin, the characterized compound, against was 198 μM in 24 h and experiment. The isolated catechin inhibited growth in both trophozoite and schizont stages. An additional experiment also suggests that the antiplasmodial property of catechin occurs through the induction of the oxidative stress to .

CONCLUSION

This result shows that the potential of catechin and its antimalarial properties should be explored further.

SUMMARY

leaf extract and fractions inhibit growthCatechin, the active compound of leaf extract, inhibits growth in a time- and dose-dependent manner RBC: Red Blood Cells; IC50: Inhibition Concentrattino 50; MeOH: Methanol; RPMI: Roswell Park Memorial Institute; EI: Electron Ionization.