Panda Bandita, Basu Bhakti, Rajaram Hema, Kumar Apte Shree
Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai, India.
Proteomics. 2014 Aug;14(16):1895-904. doi: 10.1002/pmic.201300522. Epub 2014 Jul 14.
A proteomic approach was employed to elucidate the response of an agriculturally important microbe, Anabaena sp. strain PCC7120, to methyl viologen (MV). Exposure to 2 μM MV caused 50% lethality (LD50 ) within 6 h and modified the cellular levels of several proteins. About 31 proteins increased in abundance and 24 proteins decreased in abundance, while 55 proteins showed only a minor change in abundance. Of these, 103 proteins were identified by MS. Levels of proteins involved in ROS detoxification and chaperoning activities were enhanced but that of crucial proteins involved in light and dark reactions of photosynthesis declined or constitutive. The abundance of proteins involved in carbon and energy biogenesis were altered. The study elaborated the oxidative stress defense mechanism deployed by Anabaena, identified carbon metabolism and energy biogenesis as possible major targets of MV sensitivity, and suggested potential biotechnological interventions for improved stress tolerance in Anabaena 7120.
采用蛋白质组学方法来阐明一种具有重要农业意义的微生物——鱼腥藻属PCC7120菌株对甲基紫精（MV）的反应。暴露于2 μM MV会在6小时内导致50%的致死率（LD50），并改变几种蛋白质的细胞水平。约31种蛋白质丰度增加，24种蛋白质丰度降低，而55种蛋白质仅显示出微小的丰度变化。其中，通过质谱鉴定出了103种蛋白质。参与活性氧解毒和伴侣活性的蛋白质水平增强，但参与光合作用光反应和暗反应的关键蛋白质水平下降或呈组成型。参与碳和能量生物合成的蛋白质丰度发生了改变。该研究阐述了鱼腥藻所采用的氧化应激防御机制，确定碳代谢和能量生物合成可能是MV敏感性的主要靶点，并提出了提高鱼腥藻7120胁迫耐受性的潜在生物技术干预措施。
