State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Appl Microbiol Biotechnol. 2021 Nov;105(21-22):8377-8392. doi: 10.1007/s00253-021-11628-w. Epub 2021 Oct 20.
The toxicity of methyl viologen (MV) to organisms is mainly due to the oxidative stress caused by reactive oxygen species produced from cell response. This study mainly investigated the response of Synechocystis sp. PCC 6803 to MV by combining transcriptomic and metabolomic analyses. Through transcriptome sequencing, we found many genes responding to MV stress, and analyzed them by weighted gene co-expression network analysis (WGCNA). Meanwhile, many metabolites were also found by metabolomic analysis to be regulated post MV treatment. Based on the analysis results of Kyoto encyclopedia of genes and genomes (KEGG) of the differentially expressed genes (DEGs) in the transcriptome and the differential metabolites in the metabolome, the dynamic changes of genes and metabolites involved in ten metabolic pathways in response to MV were analyzed. The results indicated that although the oxidative stress caused by MV was the strongest at 6 h, the proportion of the upregulated genes and metabolites involved in these ten metabolic pathways was the highest. Photosynthesis positively regulated the response to MV-induced oxidative stress, and the regulation of environmental information processing was inhibited by MV. Other metabolic pathways played different roles at different times and interacted with each other to respond to MV. This study comprehensively analyzed the response of Synechocystis sp. PCC 6803 to oxidative stress caused by MV from a multi-omics perspective, with providing key data and important information for in-depth analysis of the response of organisms to MV, especially photosynthetic organisms. KEY POINTS: • Methyl viologen (MV) treatment caused regulatory changes in genes and metabolites. • Proportion of upregulated genes and metabolites was the highest at 6-h MV treatment. • Photosynthesis and environmental information processing involved in MV response.
甲基紫精 (MV) 对生物的毒性主要是由于细胞反应产生的活性氧引起的氧化应激。本研究主要通过转录组学和代谢组学分析，结合研究聚球藻 PCC 6803 对 MV 的反应。通过转录组测序，我们发现了许多对 MV 应激有反应的基因，并通过加权基因共表达网络分析 (WGCNA) 对其进行了分析。同时，通过代谢组学分析还发现了许多代谢物在 MV 处理后受到调节。基于转录组中差异表达基因 (DEGs) 和代谢组中差异代谢物的京都基因与基因组百科全书 (KEGG) 分析结果，分析了十个代谢途径中参与应对 MV 的基因和代谢物的动态变化。结果表明，尽管 MV 引起的氧化应激在 6 h 时最强，但上调基因和代谢物参与这十个代谢途径的比例最高。光合作用正向调节对 MV 诱导的氧化应激的反应，而 MV 抑制了环境信息处理的调节。其他代谢途径在不同时间发挥不同的作用，并相互作用以应对 MV。本研究从多组学角度全面分析了聚球藻 PCC 6803 对 MV 引起的氧化应激的反应，为深入分析生物体对 MV 的反应，特别是光合生物体的反应，提供了关键数据和重要信息。关键点： • MV 处理引起基因和代谢物的调节变化。 • 在 MV 处理 6 小时时，上调基因和代谢物的比例最高。 • 光合作用和环境信息处理参与 MV 反应。
