Qian Yi-Xin, Kang Ji-Chuan, Lei Bang-Xing, Wang Lu, Huang Ying
Zhongguo Zhong Yao Za Zhi. 2014 Feb;39(3):438-41.
Artemisia lactiflora is an important medicinal plant in China. The antitumor and antioxidant activities of the extracts of 54 endophytic fungi from the plant were screened via MTT assay and DPPH scavenging radical assay, respectively. The bioactive strains were identified based on similarity of 5.8S gene and internal transcribed spacer (ITS) sequences. The results showed that extracts from ten (18.5%) isolates exhibited antitumor activity, and which from two (3.7%) isolates exhibited antioxidant activity. The Alternaria sp. GYBH47 strain was simultaneously having antagonistic activity against HL-60 leukemia, MCF-7 breast and COLO205 colon cell lines, and Phomopsis sp. GYBH42 strain having cytotoxic and antioxidant activities. The results indicated that endophytic fungi from Artemisia lactiflora are potential resources to find valuable bioactive components.
白花蒿是中国一种重要的药用植物。分别通过MTT法和DPPH自由基清除法对该植物54株内生真菌提取物的抗肿瘤和抗氧化活性进行了筛选。基于5.8S基因和内转录间隔区（ITS）序列的相似性对生物活性菌株进行了鉴定。结果表明，10株（18.5%）分离株的提取物具有抗肿瘤活性，2株（3.7%）分离株的提取物具有抗氧化活性。链格孢属GYBH47菌株对HL-60白血病、MCF-7乳腺癌和COLO205结肠癌细胞系同时具有拮抗活性，拟茎点霉属GYBH42菌株具有细胞毒性和抗氧化活性。结果表明，白花蒿内生真菌是寻找有价值生物活性成分的潜在资源。
