Singh Gurpreet, Katoch Archana, Razak Mod, Kitchlu Surinder, Goswami Anindya, Katoch Meenu
Microbial Biotechnology Division, Indian Institute of Integrative Medicine- CSIR, Canal Road Jammu-180001 India.
Academy of Scientific and Innovative Research, Indian Institute of Integrative Medicine- CSIR, Canal road Jammu-180001 India.
FEMS Microbiol Lett. 2017 Nov 15;364(21). doi: 10.1093/femsle/fnx194.
This study describes 32 fungal endophytes isolated from different tissues of Brugmansia aurea Lagerh. Each fungal strain was authenticated based on internal transcribed spacer rDNA sequence. Phylogenetic analysis showed that these fungi are distributed in three classes, seven orders and 12 genera. The dichloromethane extracts of endophytic strains were screened for anticancer and antimicrobial activity. Anticancer activity of extracts against human cancer cell lines revealed that 50% strains are active with IC 50 < 10 μg/mL. While analysing antimicrobial potential against both Gram-positive and Gram-negative bacteria, 56.25% endophytic strains displayed activity at least against one of the tested human pathogenic bacteria with minimum inhibitory concentration of 12.5-100 μg/mL. In vitro antagonistic activity of endophytes was analysed against Sclerotinia sp ., Aspergillus fumigatus, Fusarium solani, A. flavus and F. oxysporum pathogen . The broad-spectrum anti-phytopathogenic activity was shown by R2BA. The presence of ketoacyl synthase domain of polyketide synthase gene and high degree of bioactivity shown by endophytic fungi suggested that they have potential to produce therapeutic compounds and to serve as biocontrol agent.
本研究描述了从黄花曼陀罗不同组织中分离出的32种真菌内生菌。每个真菌菌株都基于内转录间隔区rDNA序列进行了鉴定。系统发育分析表明，这些真菌分布在3个纲、7个目和12个属中。对内生菌株的二氯甲烷提取物进行了抗癌和抗菌活性筛选。提取物对人癌细胞系的抗癌活性表明，50%的菌株具有活性，IC50<10μg/mL。在分析对革兰氏阳性菌和革兰氏阴性菌的抗菌潜力时，56.25%的内生菌株对至少一种受试人类病原菌具有活性，最低抑菌浓度为12.5 - 100μg/mL。分析了内生菌对核盘菌、烟曲霉、茄类镰刀菌、黄曲霉和尖孢镰刀菌病原体的体外拮抗活性。R2BA显示出广谱抗植物病原菌活性。聚酮合酶基因的酮酰基合酶结构域的存在以及内生真菌表现出的高度生物活性表明，它们有潜力产生治疗性化合物并作为生物防治剂。
