Lei Fangyong, Zhang Longjuan, Li Xinghua, Lin Xi, Wu Shu, Li Fengyan, Liu Junling
State Key Laboratory of Oncology in South China and Department of Radiation Oncology, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
BMC Cancer. 2014 Jun 19;14:457. doi: 10.1186/1471-2407-14-457.
Prostate tumor overexpressed 1 (PTOV1) was demonstrated to play an important role in cancer progression and was correlated with unfavorable clinical outcome. However, the clinical role of PTOV1 in cancer remains largely unknown. This study aimed to investigate the expression and clinicopathological significance of PTOV1 in breast cancer.
The mRNA and protein expression levels of PTOV1 were analyzed in 12 breast cancer cell lines and eight paired breast cancer tumors by semi-quantitative real time-PCR and western blotting, respectively. Immunohistochemistry was performed to assess PTOV1 protein expression in 169 paraffin-embedded, archived breast cancer samples. Survival analysis and Cox regression analysis were performed to investigate the clinicopathological significance of PTOV1 expression.
Our data revealed that PTOV1 was frequently overexpressed in breast cancer cell lines compared to normal human breast epithelial cells and in primary breast cancer samples compared to adjacent noncancerous breast tissues, at both the mRNA and protein levels. Moreover, high expression of PTOV1 in breast cancer is strongly associated with clinicopathological characteristics and estrogen receptor expression status (P = 0.003). Breast cancer patients with higher PTOV1 expression had substantially shorter survival times than patients with lower PTOV1 expression (P < 0.001). Univariate and multivariate analysis revealed that PTOV1 might be an independent prognostic factor for breast cancer patients (P = 0.005).
Our study showed that PTOV1 is upregulated in breast cancer cell lines and clinical samples, and its expression was positively associated with progression and aggressiveness of breast cancer, suggesting that PTOV1 could serve as an independent prognostic marker.
前列腺肿瘤过表达1（PTOV1）已被证明在癌症进展中起重要作用，且与不良临床结局相关。然而，PTOV1在癌症中的临床作用仍 largely未知。本研究旨在探讨PTOV1在乳腺癌中的表达及临床病理意义。
分别通过半定量实时PCR和蛋白质印迹法分析12种乳腺癌细胞系和8对配对乳腺癌肿瘤中PTOV1的mRNA和蛋白质表达水平。采用免疫组织化学法评估169例石蜡包埋的存档乳腺癌样本中PTOV1蛋白的表达。进行生存分析和Cox回归分析以研究PTOV1表达的临床病理意义。
我们的数据显示，与正常人乳腺上皮细胞相比，PTOV1在乳腺癌细胞系中经常过表达；与相邻非癌性乳腺组织相比，在原发性乳腺癌样本中，PTOV1在mRNA和蛋白质水平均经常过表达。此外，乳腺癌中PTOV1的高表达与临床病理特征和雌激素受体表达状态密切相关（P = 0.003）。PTOV1表达较高的乳腺癌患者的生存时间明显短于PTOV1表达较低的患者（P < 0.001）。单因素和多因素分析显示，PTOV1可能是乳腺癌患者的独立预后因素（P = 0.005）。
我们的研究表明，PTOV1在乳腺癌细胞系和临床样本中上调，其表达与乳腺癌的进展和侵袭性呈正相关，提示PTOV1可作为独立的预后标志物。
