使用偏振全内反射荧光显微镜对质膜变形和融合孔扩张进行实时研究。

Real-time investigation of plasma membrane deformation and fusion pore expansion using polarized Total Internal Reflection Fluorescence Microscopy.

作者信息

Passmore Daniel R, Rao Tejeshwar, Anantharam Arun

机构信息

Department of Biological Sciences, Wayne State University, Detroit, MI, USA.

出版信息

Methods Mol Biol. 2014;1174:263-73. doi: 10.1007/978-1-4939-0944-5_18.

DOI:10.1007/978-1-4939-0944-5_18

PMID:24947388

Abstract

Polarized Total Internal Reflection Fluorescence Microscopy (pTIRFM) allows for real-time observation of plasma membrane deformations. The technique provides insights into the dynamics of biological processes requiring rapid and localized changes in membrane shape. Such processes include exocytosis, endocytosis, cytokinesis, and cell motility. In this chapter, we describe how to implement a polarization-based TIRF imaging system to monitor exocytosis in adrenal chromaffin cells.

摘要

偏振全内反射荧光显微镜（pTIRFM）能够实时观察质膜变形。该技术为深入了解需要膜形状快速且局部变化的生物过程的动力学提供了思路。此类过程包括胞吐作用、胞吞作用、胞质分裂和细胞运动。在本章中，我们将描述如何搭建一个基于偏振的全内反射荧光成像系统来监测肾上腺嗜铬细胞中的胞吐作用。