Mylona Eleni, Melissaris Savvas, Nomikos Alexandros, Theohari Irene, Giannopoulou Ioanna, Tzelepis Konstantinos, Nakopoulou Lydia
5th Department of Internal Medicine, Evagelismos Hospital, Athens, Greece.
1st Department of Pathology, Medical School, University of Athens, Athens, Greece.
Pathol Res Pract. 2014 Aug;210(8):533-40. doi: 10.1016/j.prp.2014.05.009. Epub 2014 May 23.
Our purpose was to investigate the expression pattern of BRCA1 protein in sporadic breast carcinomas, as well as the clinicopathological and prognostic value of its subcellular localizations. Immunohistochemistry was performed on paraffin embedded tissue specimens from 111 sporadic, invasive breast carcinomas to detect the expression of the proteins BRCA1, ER, PR, erbB2, p53 and Ki67. BRCA1 protein was detected in the nuclei and the cytoplasm of the tumor cells. Nuclear BRCA1 immunoreactivity showed no relation with the classic clinicopathological markers and the expression of cerbB2, p53 and Ki67. Reduced expression of nuclear BRCA1 protein was found to exert an independent favorable impact on both the overall and relapse-free (RF) survival of the patients (p=0.019 and p=0.043, respectively). Cytoplasmic BRCA1 was associated with none of the classic histomorphological indices, except from the lymph node metastasis, with which its relation was found to be inverse (p=0.05), prolonging the RF survival of the patients (p=0.05). Our findings suggest that BRCA1 protein depicts different prognostic significance, according to its subcellular distribution. Nuclear detection of the protein was associated with a worse prognosis, while the cytoplasmic one was related to fewer recurrences as a result of fewer lymph node metastases.
我们的目的是研究BRCA1蛋白在散发性乳腺癌中的表达模式，以及其亚细胞定位的临床病理和预后价值。对111例散发性浸润性乳腺癌石蜡包埋组织标本进行免疫组织化学检测，以检测BRCA1、雌激素受体（ER）、孕激素受体（PR）、表皮生长因子受体2（erbB2）、p53和Ki67蛋白的表达。在肿瘤细胞核和细胞质中检测到BRCA1蛋白。细胞核BRCA1免疫反应性与经典临床病理标志物以及erbB2、p53和Ki67的表达均无关联。发现细胞核BRCA1蛋白表达降低对患者的总生存期和无复发生存期均有独立的有利影响（分别为p = 0.019和p = 0.043）。细胞质BRCA1与除淋巴结转移外的所有经典组织形态学指标均无关联，发现其与淋巴结转移呈负相关（p = 0.05），并延长了患者的无复发生存期（p = 0.05）。我们的研究结果表明，BRCA1蛋白根据其亚细胞分布具有不同的预后意义。该蛋白的细胞核检测与较差的预后相关，而细胞质检测则与较少的复发相关，这是由于较少的淋巴结转移所致。
