Xing Huayi, Zhou Mouwang, Assinck Peggy, Liu Nan
Department of Rehabilitation Medicine, Peking University Third Hospital, 49 North Garden Road, Beijing, 100191, PR China.
Muscle Nerve. 2015 Mar;51(3):400-11. doi: 10.1002/mus.24322. Epub 2015 Jan 10.
Electrical stimulation is often used to prevent muscle atrophy and preserve contractile function, but its effects on the satellite cell population after nerve injury are not well understood. In this study we aimed to determine whether satellite cell differentiation is affected by electrical stimulation after nerve crush.
The sciatic nerves of Sprague-Dawley (SD) rats were crushed. Half of the injured rats received daily electrical stimulation of the gastrocnemius muscle, and the others did not. Tests for detecting paired box protein 7 (Pax7), myogenic differentiation antigen (MyoD), embryonic myosin heavy chain (eMyHC), and force production were performed 2, 4, and 6 weeks after injury.
More Pax7+/MyoD+ nuclei in stimulated muscles were observed than in non-stimulated muscles. eMyHC expression was elevated in stimulated muscles and correlated positively with enhanced force production.
Increased satellite cell differentiation is correlated with preserved muscle function in response to electrical stimulation after nerve injury.
电刺激常用于预防肌肉萎缩并维持收缩功能，但电刺激对神经损伤后卫星细胞群体的影响尚不清楚。在本研究中，我们旨在确定神经挤压后卫星细胞分化是否受电刺激影响。
对Sprague-Dawley（SD）大鼠的坐骨神经进行挤压。一半受伤大鼠每天接受腓肠肌电刺激，另一半则不接受。在损伤后2、4和6周进行检测配对盒蛋白7（Pax7）、生肌分化抗原（MyoD）、胚胎肌球蛋白重链（eMyHC）和力量产生的测试。
与未受刺激的肌肉相比，在受刺激的肌肉中观察到更多Pax7+/MyoD+核。eMyHC表达在受刺激的肌肉中升高，并且与增强的力量产生呈正相关。
神经损伤后，卫星细胞分化增加与电刺激后肌肉功能的保留相关。
