Pagarete António, Kusonmano Kanthida, Petersen Kjell, Kimmance Susan A, Martínez Martínez Joaquín, Wilson William H, Hehemann Jan-Hendrik, Allen Michael J, Sandaa Ruth-Anne
Department of Biology, University of Bergen, Norway.
Computational Biology Unit, University of Bergen, Norway.
Virology. 2014 Oct;466-467:129-37. doi: 10.1016/j.virol.2014.05.020. Epub 2014 Jun 16.
Despite the global oceanic distribution and recognised biogeochemical impact of coccolithoviruses (EhV), their diversity remains poorly understood. Here we employed a metagenomic approach to study the occurrence and progression of natural EhV community genomic variability. Analysis of EhV metagenomes from the early and late stages of an induced bloom led to three main discoveries. First, we observed resilient and specific genomic signatures in the EhV community associated with the Norwegian coast, which reinforce the existence of limitations to the capacity of dispersal and genomic exchange among EhV populations. Second, we identified a hyper-variable region (approximately 21kbp long) in the coccolithovirus genome. Third, we observed a clear trend for EhV relative amino-acid diversity to reduce from early to late stages of the bloom. This study validated two new methodological combinations, and proved very useful in the discovery of new genomic features associated with coccolithovirus natural communities.
尽管球石藻病毒（EhV）具有全球海洋分布并已被认可的生物地球化学影响，但其多样性仍知之甚少。在此，我们采用宏基因组学方法研究自然EhV群落基因组变异性的发生和演变。对诱导藻华早期和晚期的EhV宏基因组进行分析得出了三个主要发现。第一，我们在与挪威海岸相关的EhV群落中观察到了有弹性且特定的基因组特征，这强化了EhV种群间传播能力和基因组交换存在限制的观点。第二，我们在球石藻病毒基因组中鉴定出一个高变区（约21kbp长）。第三，我们观察到一个明显的趋势，即EhV相对氨基酸多样性从藻华早期到晚期逐渐降低。本研究验证了两种新的方法组合，并在发现与球石藻病毒自然群落相关的新基因组特征方面非常有用。
