Verma Poonam, Gauttam Vinod, Kalia Ajudhia N
Department of Pharmacognosy, Indo Soviet Friendship College of Pharmacy, Moga, Punjab, India.
J Ayurveda Integr Med. 2014 Apr;5(2):104-8. doi: 10.4103/0975-9476.131728.
Pashanbhed is a commercially available diuretic and lithotropic drug, used to treat renal problems. It is a controversial name as it is assigned to various plants such as Bergenia ligulata, Kalanchoe pinnata, Coleus aromaticus and Rotula aquatica.
To perform the comparative preliminary phytochemical screening, diuretic activity, and thin layer chromatography (TLC) finger printing profile of three plants (B. ligulata, C. aromaticus, and K. pinnata), most commonly used as Pashanbhed.
Diuretic potential of methanolic extract (ME) of three plants were evaluated at two dose levels (500 and 1,000 mg/kg p.o.), using normal Wistar rats (Lipschitz method). Furosemide (20 mg/kg p.o.) was used as a standard drug. The effect on urine output and electrolyte changes were measured for 24 h and compared. All MEs were screened preliminarily for their constituents and their TLC finger printing profiles were prepared. One-way analysis of variance (ANOVA) followed by Bonferroni's multiple comparison test. P < 0.05 was considered statistically significant.
The MEs of all three plants have shown diuresis in normal rats. However, in intercomparison of the ME C. aromaticus (1,000 mg/kg p.o.) produced more significant diuresis (P < 0.05) and electrolyte excretion compared to other test groups, the effect was at par with furosemide. The ME of these plants showed presence of alkaloids, glycosides, steroids, terpenoids, saponins, flavonoids, etc.
The ME of C. aromaticus (1,000 mg/kg p.o.) has showed highest diuretic action (4.2) among the tested extracts. This suggests the use of C. aromaticus leaves as "Pashanbhed"; the most effective diuretic drug.
帕山贝德是一种市面上可买到的利尿和溶石药物，用于治疗肾脏问题。它是一个有争议的名称，因为它被用于多种植物，如岩白菜、落地生根、芳香鞘蕊花和水生旋蒴苣苔。
对三种最常被用作帕山贝德的植物（岩白菜、芳香鞘蕊花和落地生根）进行比较性初步植物化学筛选、利尿活性及薄层色谱（TLC）指纹图谱分析。
采用正常Wistar大鼠（利普希茨法），在两个剂量水平（500和1000毫克/千克口服）评估三种植物甲醇提取物（ME）的利尿潜力。呋塞米（20毫克/千克口服）用作标准药物。测量24小时内对尿量和电解质变化的影响并进行比较。对所有ME进行成分初步筛选并制备其TLC指纹图谱。采用单因素方差分析（ANOVA），随后进行邦费罗尼多重比较检验。P<0.05被认为具有统计学意义。
所有三种植物的ME在正常大鼠中均显示出利尿作用。然而，相互比较时，芳香鞘蕊花ME（1000毫克/千克口服）与其他测试组相比产生了更显著的利尿作用（P<0.05）和电解质排泄，其效果与呋塞米相当。这些植物的ME显示含有生物碱、糖苷、类固醇、萜类、皂苷、黄酮类等。
在测试提取物中，芳香鞘蕊花ME（1000毫克/千克口服）显示出最高的利尿作用（4.2）。这表明可将芳香鞘蕊花的叶子用作“帕山贝德”，即最有效的利尿药物。
