不同类型芭蕉假茎提取物抑制草酸钙结晶的潜在抗尿路结石活性研究。
Investigation of potential anti-urolithiatic activity from different types of Musa pseudo-stem extracts in inhibition of calcium oxalate crystallization.
作者信息
Abu Zarin Mazni, Tan Joo Shun, Murugan Paramasivam, Ahmad Rosma
机构信息
Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800, Gelugor, Pulau Pinang, Malaysia.
Laboratory Vaccines and Immunotherapeuthics, Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, 43400, Serdang, Selangor, Malaysia.
出版信息
BMC Complement Med Ther. 2020 Oct 19;20(1):317. doi: 10.1186/s12906-020-03113-0.
BACKGROUND
The banana or scientifically referred to as Musa sp., is one of the most popular fruits all over the world. Almost all parts of a banana tree, including the fruits, stem juice, and flowers are commonly used as traditional medicine for treating diarrhoea (unripe), menorrhagia, diabetes, dysentery, and antiulcerogenic, hypoglycemic, antilithic, hypolipidemic conditions, plus antioxidant actions, inflammation, pains and even snakebites. The study carried out was to evaluate in vitro anti-urolithiatic activity from different types of Musa pseudo-stems.
METHODS
Observing anti-urolithiathic activity via in vitro nucleation and aggregation assay using a spectrophotometer followed by microscopic observation. A total of 12 methanolic extracts were tested to determine the potential extracts in anti-urolithiasis activities. Cystone was used as a positive control.
RESULTS
The results manifested an inhibition of nucleation activity (0.11 ± 2.32% to 55.39 ± 1.01%) and an aggregation activity (4.34 ± 0.68% to 58.78 ± 1.81%) at 360 min of incubation time. The highest inhibition percentage in nucleation assay was obtained by the Musa acuminate x balbiciana Colla cv "Awak Legor" methanolic pseudo-stem extract (2D) which was 55.39 ± 1.01%at 60 min of incubation time compared to the cystone at 30.87 ± 0.74%. On the other hand,the Musa acuminate x balbiciana Colla cv "Awak Legor" methanolic bagasse extract (3D) had the highest inhibition percentage in the aggregation assay incubated at 360 min which was obtained at 58.78 ± 1.8%; 5.53% higher than the cystone (53.25%).The microscopic image showed a great reduction in the calcium oxalate (CaOx) crystals formation and the size of crystals in 2D and 3D extracts, respectively, as compared to negative control.
CONCLUSIONS
The results obtained from this study suggest that the extracts are potential sources of alternative medicine for kidney stones disease.
背景
香蕉，科学上称为芭蕉属（Musa sp.），是全球最受欢迎的水果之一。香蕉树的几乎所有部分，包括果实、茎汁和花朵，通常都被用作传统药物来治疗腹泻（未成熟果实）、月经过多、糖尿病、痢疾，以及具有抗溃疡、降血糖、抗结石、降血脂作用，还有抗氧化、抗炎、止痛甚至治疗蛇咬伤的功效。本研究旨在评估不同类型香蕉假茎的体外抗结石活性。
方法
使用分光光度计通过体外成核和聚集试验观察抗结石活性，随后进行显微镜观察。共测试了12种甲醇提取物，以确定其在抗结石活性方面的潜在提取物。胱氨酸用作阳性对照。
结果
结果表明，在孵育360分钟时，成核活性受到抑制（从0.11±2.32%至55.39±1.01%），聚集活性也受到抑制（从4.34±0.68%至58.78±1.81%）。在成核试验中，尖叶蕉×大蕉（Musa acuminate x balbiciana Colla）品种“Awak Legor”的甲醇假茎提取物（2D）在孵育60分钟时获得了最高抑制率，为55.39±1.01%，而胱氨酸为30.87±0.74%。另一方面，尖叶蕉×大蕉品种“Awak Legor”的甲醇蔗渣提取物（3D）在孵育360分钟的聚集试验中获得了最高抑制率，为58.78±1.8%；比胱氨酸（53.25%）高5.53%。显微镜图像显示，与阴性对照相比，2D和3D提取物中草酸钙（CaOx）晶体的形成和晶体大小分别大幅减少。
结论
本研究结果表明，这些提取物是肾结石疾病替代药物的潜在来源。
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