Cui Peng, He Xiaohong, Pu Yabin, Zhang Wenxiu, Zhang Ping, Li Changli, Guan Weijun, Li Xiangchen, Ma Yuehui
Institute of Beijing Animal Science and Veterinary, Chinese Academy of Agricultural Science, Beijing 100194, China.
Institute of Beijing Animal Science and Veterinary, Chinese Academy of Agricultural Science, Beijing 100194, China ; College of Pharmacy, Jiamusi University, Heilongjiang Province Key Laboratory of Biological Medicine Formulation, Jiamusi, Heilongjiang 154007, China.
Biomed Res Int. 2014;2014:786234. doi: 10.1155/2014/786234. Epub 2014 May 18.
Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were a multipotential stem cell population, which has potential applications in the tissue damage repair and skin transplant. Although a large number of studies deal with the human DMS/PCs self-renewal and regulation, however, the study of livestock-derived DMS/PCs has rarely been reported. Here, sheep DMS/PCs were isolated from one-month-old sheep embryos and studied at the cellular and molecular level. And then the DMS/PCs biological characteristics were analysed by RT-PCR and immunofluorescence. Experimental results showed that DMS/PCs could be expanded for 48 passages and the cells viability and hereditary character were steady. In addition, the DMS/PCs maker β -integrin, CD71, CD44, and CD73 were expressed positively through RT-PCR and immunofluorescence. Passage 3 DMS/PCs were successfully induced to differentiate into adipocytes, osteoblasts, chondrocytes, and neurocytes, respectively. The above results suggest that DMS/PCs not only have strong self-renewal capacity but also have the potential to differentiate into adipocytes, osteoblasts, chondrocytes, and neurocytes. The study provides theoretical basis and experimental evidence for potential clinical application.
真皮来源的间充质干/祖细胞（DMS/PCs）是一种多能干细胞群体，在组织损伤修复和皮肤移植方面具有潜在应用价值。尽管大量研究涉及人类DMS/PCs的自我更新和调控，然而，关于家畜来源的DMS/PCs的研究却鲜有报道。在此，从1月龄绵羊胚胎中分离出绵羊DMS/PCs，并在细胞和分子水平上进行研究。然后通过逆转录聚合酶链反应（RT-PCR）和免疫荧光分析DMS/PCs的生物学特性。实验结果表明，DMS/PCs可传代培养48代，细胞活力和遗传特性稳定。此外，通过RT-PCR和免疫荧光检测发现，DMS/PCs标志物β-整合素、CD71、CD44和CD73呈阳性表达。第3代DMS/PCs分别成功诱导分化为脂肪细胞、成骨细胞、软骨细胞和神经细胞。上述结果表明，DMS/PCs不仅具有强大的自我更新能力，而且具有分化为脂肪细胞、成骨细胞、软骨细胞和神经细胞的潜力。该研究为其潜在的临床应用提供了理论依据和实验证据。
