Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
School of kinesiology and health, Harbin Institute of Physical Education, Harbin, Heilongjiang province 150008, China.
Poult Sci. 2018 Sep 1;97(9):3236-3247. doi: 10.3382/ps/pey178.
The skin is a natural target of stem cell research because of its large size and easy accessibility. Cutaneous mesenchymal stem cells have shown to be a promising source of various adult stem cell or progenitor cell populations, which provide an important source of stem cell-based investigation. Nowadays, much work has been done on dermal-derived mesenchymal stem cells (DMSCs) from humans, mice, sheep, and other mammals, but the literature on avian species has been rarely reported. As an animal model, the goose is an endemic species abounding in dermal tissues which is important in the global economy. In this study, we isolated and established the mesenchymal stem cell line from dermis tissue of goose, which were subcultured to passage 21 in vitro without loss of their functional integrity in terms of morphology, renewal capacity, and presence of mesenchymal stem cell markers. Cryopreservation and resuscitation were also observed in different passages. To investigate the biological characteristics of goose DMSCs, immunofluorescence, reverse transcription-polymerase chain reaction, and flow cytometry were used to detect the characteristic surface markers. Growth curves and the capacity of colony forming were performed to test the self-renew and proliferative ability. Furthermore, the DMSCs are induced to osteoblasts, adipocytes, and chondrocytes in vitro. Our results suggest that DMSCs isolated from goose embryos possess similar biological characteristics to those from other species. The methods in establishment and cultivation of goose DMSCs line demonstrated a good self-renew and expansion potential in vitro, which provided a technological platform for preserving the valuable genetic resources of poultry and a great inspiration for in vitro investigation of avian MSCs.
皮肤是干细胞研究的天然靶标，因为其体积大且易于接近。皮肤间充质干细胞已被证明是各种成体干细胞或祖细胞群体的有前途的来源，为基于干细胞的研究提供了重要的来源。如今，已经对来自人类、小鼠、绵羊和其他哺乳动物的真皮衍生间充质干细胞（DMSCs）进行了大量研究，但有关禽类物种的文献却很少报道。作为一种动物模型，鹅是一种特有的物种，其皮肤组织丰富，对全球经济具有重要意义。在这项研究中，我们从鹅的真皮组织中分离并建立了间充质干细胞系，该细胞系在体外传代至第 21 代，在形态、更新能力和间充质干细胞标志物的存在方面均未丧失其功能完整性。还观察了不同传代中的冷冻保存和复苏。为了研究鹅 DMSC 的生物学特性，我们使用免疫荧光、逆转录-聚合酶链反应和流式细胞术检测了特征性表面标志物。进行生长曲线和集落形成能力测定，以测试自我更新和增殖能力。此外，还将 DMSC 体外诱导为成骨细胞、脂肪细胞和成软骨细胞。我们的结果表明，从鹅胚胎中分离的 DMSC 具有与其他物种相似的生物学特性。鹅 DMSC 系的建立和培养方法在体外显示出良好的自我更新和扩增潜力，为禽类遗传资源的保存提供了技术平台，也为禽类 MSC 的体外研究提供了重要启示。
