分析真皮中细胞表面标志物的细胞特征。
Analysis of cell characterization using cell surface markers in the dermis.
机构信息
Research Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimicho, Nishi-Ku, Nagoya, Aichi, 451-0071, Japan.
出版信息
J Dermatol Sci. 2011 May;62(2):98-106. doi: 10.1016/j.jdermsci.2011.01.012. Epub 2011 Feb 15.
BACKGROUND
In recent years, it has been reported that stem cells exist in the mesenchymal tissues of the bone marrow and adipose. These stem cells are thought to express specific cell surface markers such as CD44, CD54, CD105, CD90, and CD271 and have been confirmed to be pluripotent. Furthermore, although it has been reported that stem cells are also present in the dermis, their cell surface markers and characteristics are not fully understood.
OBJECTIVE
To confirm the presence of stem cells in the dermis and their ability, employing the mesenchymal stem cell markers which have previously been reported as an indication.
METHODS
We analyzed the percentages of CD44 (+), CD54 (+), CD90 (+), CD105 (+), and CD271 (+) cells in the dermis of neonatal mice (HR-1 mouse) by performing immunostaining and FACS. Secondly, we isolated each type of marker-positive and -negative cells from dermal tissues and evaluated their proliferation potential and their ability to differentiate into adipocytes, osteoblasts, and chondrocytes.
RESULTS
According to the immunostaining and FACS results, we confirmed that stem cells that express CD44, CD54, CD90, CD105, and CD271 are present in the dermal tissues of neonatal mice. In addition, when we measured the proliferation and differentiation potentials of each type of marker-positive cells, it was revealed that cells expressing CD54 or CD271 have a high proliferation potential and are able to differentiate into adipocytes, osteoblasts, and chondrocytes.
CONCLUSIONS
These results indicated that dermal tissues contain stem cells that express CD44, CD54, CD90, CD105, and CD271 which are stem cell markers. More precisely, it was suggested that both CD54 (+) and CD271 (+) stem cells have high proliferation and differentiation potentials.
背景
近年来，有报道称骨髓和脂肪的间充质组织中存在干细胞。这些干细胞被认为表达特定的细胞表面标志物，如 CD44、CD54、CD105、CD90 和 CD271，并已被证实具有多能性。此外，尽管有报道称真皮中也存在干细胞，但它们的细胞表面标志物和特性尚未完全了解。
目的
通过使用先前报道的间充质干细胞标志物来证实真皮中存在干细胞及其能力。
方法
我们通过免疫染色和 FACS 分析了新生小鼠（HR-1 小鼠）真皮中 CD44(+)、CD54(+)、CD90(+)、CD105(+)和 CD271(+)细胞的百分比。其次，我们从真皮组织中分离出每种标记阳性和阴性的细胞，并评估它们的增殖潜力和分化为脂肪细胞、成骨细胞和成软骨细胞的能力。
结果
根据免疫染色和 FACS 结果，我们证实了表达 CD44、CD54、CD90、CD105 和 CD271 的干细胞存在于新生小鼠的真皮组织中。此外，当我们测量每种标记阳性细胞的增殖和分化潜力时，发现表达 CD54 或 CD271 的细胞具有较高的增殖潜力，并且能够分化为脂肪细胞、成骨细胞和成软骨细胞。
结论
这些结果表明真皮组织中含有表达 CD44、CD54、CD90、CD105 和 CD271 的干细胞，这些是干细胞标志物。更确切地说，CD54(+)和 CD271(+)干细胞具有较高的增殖和分化潜力。
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