Shakhbazau Antos, Mohanty Chandan, Kumar Ranjan, Midha Rajiv
Department of Clinical Neuroscience, Faculty of Medicine.
J Neurosurg. 2014 Aug;121(2):423-31. doi: 10.3171/2014.5.JNS132132. Epub 2014 Jun 20.
Cell therapy is a promising candidate among biological or technological innovations sought to augment microsurgical techniques in peripheral nerve repair. This report describes long-term functional regenerative effects of cell therapy in the rat injury model with a focus on sensory recovery.
Schwann cells were derived from isogenic nerve or skin precursor cells and injected into the transected and immediately repaired sciatic nerve distal to the injury site. Sensory recovery was assessed at weeks 4, 7, and 10. Axonal regeneration was assessed at Week 11.
By Week 10, thermal sensitivity in cell therapy groups returned to a level indistinguishable from the baseline (p > 0.05). Immunohistochemistry at 11 weeks after injury showed improved regeneration of NF+ and IB4+ axons.
The results of this study show that cell therapy significantly improves thermal sensation and the number of regenerated sensory neurons at 11 weeks after injury. These findings contribute to the view of skin-derived stem cells as a reliable source of Schwann cells with therapeutic potential for functional recovery in damaged peripheral nerve.
在旨在增强外周神经修复显微外科技术的生物或技术创新中，细胞疗法是一个有前景的选择。本报告描述了细胞疗法在大鼠损伤模型中的长期功能再生效应，重点关注感觉恢复。
雪旺细胞源自同基因神经或皮肤前体细胞，并注入到损伤部位远端横断并立即修复的坐骨神经中。在第4、7和10周评估感觉恢复情况。在第11周评估轴突再生情况。
到第10周时，细胞治疗组的热敏感性恢复到与基线无显著差异的水平（p>0.05）。损伤后11周的免疫组织化学显示NF+和IB4+轴突的再生有所改善。
本研究结果表明，细胞疗法在损伤后11周时显著改善了热感觉和再生感觉神经元的数量。这些发现有助于将皮肤来源的干细胞视为雪旺细胞的可靠来源，其对受损外周神经的功能恢复具有治疗潜力。
