Shah P, van der Meide P H, Borman T, Schroeder N, Bliss J M, Coombes R C
Ludwig Institute for Cancer Research, St George's Hospital Medical School, London, UK.
Br J Cancer. 1989 Feb;59(2):206-9. doi: 10.1038/bjc.1989.42.
We have used the nitrosomethylurea-induced rat mammary tumour model to study the effects of parenteral administration of human recombinant tumour necrosis factor (rHu-TNF) and rat gamma interferon (IFN-gamma). An inbred strain of tumour bearing female Ludwig/Wistar/Olac rats were randomised to either treatment or control groups. Two independent studies showed that combined treatment with rHu-TNF and rat IFN-gamma induced significant tumour regression over 4 weeks (P = 0.004, P = 0.005 respectively). Treatment with either rHu-TNF or rat IFN-gamma given individually did not affect the overall rate of tumour growth (P = 0.157 and 0.40 respectively) although an initial reduction in tumour size was observed during the first few days after injection. Measurement of circulating oestradiol levels in groups in which maximum tumour regression was observed showed no statistically significant difference when compared to the control group. Similarly, measurement of oestrogen receptor content showed no statistically significant difference between rHu-TNF-gamma or rat-IFN-gamma treatment or combined treatment of rHu-TNF and IFN-gamma with the control group. We conclude from these observations that combined therapy with rHu-TNF and rat IFN-gamma may prove to be useful new forms of treatment for human breast cancer.
我们利用亚硝基甲基脲诱导的大鼠乳腺肿瘤模型，研究了肠胃外注射人重组肿瘤坏死因子（rHu-TNF）和大鼠γ干扰素（IFN-γ）的效果。将患有肿瘤的近交系雌性路德维希/威斯塔/奥拉大鼠随机分为治疗组和对照组。两项独立研究表明，rHu-TNF和大鼠IFN-γ联合治疗在4周内可显著诱导肿瘤消退（分别为P = 0.004，P = 0.005）。单独使用rHu-TNF或大鼠IFN-γ治疗均未影响肿瘤的总体生长速度（分别为P = 0.157和0.40），尽管在注射后的头几天观察到肿瘤大小有初步减小。在观察到最大肿瘤消退的组中，测量循环雌二醇水平，与对照组相比无统计学显著差异。同样，测量雌激素受体含量，rHu-TNF-γ或大鼠IFN-γ治疗组以及rHu-TNF和IFN-γ联合治疗组与对照组之间均无统计学显著差异。基于这些观察结果，我们得出结论，rHu-TNF和大鼠IFN-γ联合治疗可能被证明是治疗人类乳腺癌的有用新方法。
