Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University, Mollasani, Ahvaz, Iran.
Int J Biol Macromol. 2014 Aug;69:523-31. doi: 10.1016/j.ijbiomac.2014.06.008. Epub 2014 Jun 17.
The objective of the present research was to investigate the effect of microwave-assisted extraction (MAE) conditions on the extraction yield, antioxidant properties and antilisterial activities of the polysaccharides from the flowers of viper's bugloss (Echium vulgare L.). The four extraction variables, time (40-100min), microwave power (200-800W), temperature (30-70°C), and the ratio of water to raw material (10-70), were optimized using response surface methodology. The experimental data were matched to a second-order polynomial equation. The optimal conditions for MAE of polysaccharides (EVFP) were time 73.8min, microwave power 769.2W, temperature 42.3°C and the ratio of water to raw material 61.4, where the actual yield of EVFP 25.11±0.87% was obtained (versus the value predicted by the model 25.36%). The results indicated that EVFP has significant radical (·OH and ·DPPH) scavenging abilities in vitro assay. Moreover, the antilisterial activity was confirmed against four species of Listeria. EVFP, at a concentration of 5mg/mL, demonstrated great antilisterial properties against Listeria ivanovii and Listeria monocytogenes, with inhibition zones of 10.76±0.32mm and 8.64±0.47mm, respectively.
本研究旨在探讨微波辅助提取（MAE）条件对琉璃苣花多糖提取率、抗氧化性能和抗李斯特菌活性的影响。通过响应面法优化了提取时间（40-100min）、微波功率（200-800W）、温度（30-70°C）和料液比（10-70）这四个提取变量。实验数据与二次多项式方程相匹配。MAE 提取多糖（EVFP）的最佳条件为：时间 73.8min、微波功率 769.2W、温度 42.3°C、料液比 61.4，在此条件下 EVFP 的实际产率为 25.11±0.87%（与模型预测值 25.36%相比）。结果表明，EVFP 在体外试验中具有显著的自由基（·OH 和·DPPH）清除能力。此外，还证实了其对四种李斯特菌的抗李斯特菌活性。在 5mg/mL 浓度下，EVFP 对李斯特菌 ivanovii 和李斯特菌 monocytogenes 具有很强的抗李斯特菌特性，抑菌圈分别为 10.76±0.32mm 和 8.64±0.47mm。
