Zhang Anqiang, Li Xueqing, Xing Chen, Yang Junhong, Sun Peilong
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
Int J Biol Macromol. 2014 Apr;65:28-32. doi: 10.1016/j.ijbiomac.2014.01.013. Epub 2014 Jan 10.
Ultrasonic technology was applied for polysaccharide extraction from the fruiting bodies of P. Eryngii, and response surface methodology (RSM) was used to optimize the effects of processing parameters on polysaccharide extraction yield. Three independent variables were extraction time (A), ultrasonic power (B) and the ratio of solvent to sample (C), respectively. Results showed that the maximum yield of P. eryngii polysaccharide (PEPS) was obtained at an optimum condition: extraction time 39 min, ultrasonic power 517 W, the ratio of water to material 19 mL/g, and the PEPS extracting yield reached 34.3% under the conditions. PEPS were precipitated into three crude polysaccharides, PEPS30, PEPS60 and PEPS80, by different concentrations of ethanol respectively. The antioxidant activities of these three polysaccharides were evaluated. The results showed that PEPS80 had the best reducing power, DPPH radical scavenging ability and oxygen radical scavenging ability followed by PEPS60 and PEPS30.
采用超声技术从杏鲍菇子实体中提取多糖，并运用响应面法（RSM）优化工艺参数对多糖提取率的影响。三个自变量分别为提取时间（A）、超声功率（B）和溶剂与样品的比例（C）。结果表明，在最佳条件下可获得杏鲍菇多糖（PEPS）的最大产量：提取时间39分钟、超声功率517瓦、水料比19毫升/克，在此条件下PEPS提取率达到34.3%。分别用不同浓度的乙醇将PEPS沉淀为三种粗多糖，即PEPS30、PEPS60和PEPS80。对这三种多糖的抗氧化活性进行了评估。结果表明，PEPS80具有最佳的还原力、DPPH自由基清除能力和氧自由基清除能力，其次是PEPS60和PEPS30。
