Department of Forestry Pathology, College of Forestry, Northwest Agricultural & Forestry University, No. 3, Taicheng Road, Yangling 712100, Shanxi, China.
MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Int J Biol Macromol. 2015 Jan;72:19-27. doi: 10.1016/j.ijbiomac.2014.07.057. Epub 2014 Aug 9.
Polysaccharide prepared from pericarp of Zanthoxylum bungeanum was proved to possess excellent antioxidant activities in vitro by using reducing ferric iron power, DPPH radical scavenging activity, chelating ferrous iron capacity, and hydroxyl radical scavenging activity assays in the present study. In those four antioxidant assay models, Z. bungeanum polysaccharide (ZBP) displayed prominent antioxidant activities with low EC50 values of 0.011, 0.021, 0.056 and 0.008 mg/mL, respectively. Moreover, the extraction process of ZBP was further optimized by response surface methodology combined with Box-Behnken design. The highest polysaccharide yield 13.96%, which agreed closely with the predicted yield 13.20%, was obtained under the optimal extraction conditions as follows: extraction temperature 89 °C, extraction time 3h, ratio of water volume (mL) to raw material weight (g) 29 (v/w), and extraction number two times. The present research not only provide theoretical basis for exploitation of natural polysaccharide antioxidants, but also establish the foundation of large-scale production and further system utilization of ZBP.
从花椒果皮中提取的多糖通过还原铁离子能力、DPPH 自由基清除活性、亚铁离子螯合能力和羟基自由基清除活性测定，被证明具有优异的体外抗氧化活性。在这四种抗氧化测定模型中，花椒多糖（ZBP）表现出突出的抗氧化活性，其 EC50 值分别为 0.011、0.021、0.056 和 0.008mg/mL。此外，通过响应面法结合 Box-Behnken 设计进一步优化了 ZBP 的提取工艺。在最佳提取条件下，多糖得率为 13.96%，与预测值 13.20%非常接近，最佳提取条件为：提取温度 89°C，提取时间 3h，料液比（mL/g）29，提取次数 2 次。本研究不仅为天然多糖类抗氧化剂的开发提供了理论依据，而且为 ZBP 的大规模生产和进一步系统利用奠定了基础。
