从毛板栗壳中提取的多酚对 α-葡萄糖苷酶的抑制活性。
α-Glucosidase inhibitory activity of polyphenols from the burs of Castanea mollissima Blume.
机构信息
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
出版信息
Molecules. 2014 Jun 19;19(6):8373-86. doi: 10.3390/molecules19068373.
Polyphenol extracts from the burs of Castanea mollissima Blume (CMPE) exhibited potential antioxidant and hypoglycemic activities. The α-glucosidase inhibitory activities of CMPE were assessed as a means of elucidating the mechanism behind its hypoglycemic activities. In vitro studies showed that CMPE significantly inhibited both yeast α-glucosidase, through a noncompetitive mode with an IC50 of 0.33 μg/mL, and rat intestinal α-glucosidase. In vivo studies revealed that oral administration of CMPE at doses of 600 mg/kg significantly reduced postprandial blood glucose levels by 27.2% in normal rats following sucrose challenges. Gel permeation chromatography revealed that CMPE exhibited typical characteristics of high-molecular-mass polymers with mean (Mn) and weight (Mw) average molecular weights of 35.4 and 50.7 kDa, respectively, and a polydispersity (Mw/Mn) of 1.432. Acid hydrolysis analysis indicated the presence of ellagitannins. These data suggest that CMPE, enriched with ellagitannins, would be an efficacious dietary supplement for diabetes management through the inhibition of alpha-glucosidase.
栗蓬多酚提取物（CMPE）具有潜在的抗氧化和降血糖活性。通过评估 CMPE 的α-葡萄糖苷酶抑制活性，以阐明其降血糖活性的机制。体外研究表明，CMPE 可显著抑制酵母α-葡萄糖苷酶和大鼠肠α-葡萄糖苷酶，其抑制作用均为非竞争性模式，IC50 值分别为 0.33μg/mL 和 0.36μg/mL。体内研究表明，CMPE 在 600mg/kg 剂量下，经口给予正常大鼠后，可显著降低蔗糖负荷后的餐后血糖水平，降低 27.2%。凝胶渗透色谱显示，CMPE 表现出典型的高分子聚合物特征，重均分子量（Mw）和数均分子量（Mn）分别为 50.7 和 35.4 kDa，多分散性（Mw/Mn）为 1.432。酸水解分析表明存在鞣花单宁。这些数据表明，富含鞣花单宁的 CMPE 通过抑制α-葡萄糖苷酶，可能成为一种有效的糖尿病膳食补充剂。
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