Jo Sung-Hoon, Cho Cha-Young, Lee Jung-Yoon, Ha Kyoung-Soo, Kwon Young-In, Apostolidis Emmanouil
Department of Chemistry and Food Science, Framingham State University, Framingham, MA, 01701, USA.
Department of Food and Nutrition, Hannam University, Daejeon, 305-811, South Korea.
BMC Complement Altern Med. 2016 Mar 31;16:111. doi: 10.1186/s12906-016-1090-4.
Type 2 diabetes is a serious problem for developed and developing countries. Prevention of prediabetes progression to type 2 diabetes with the use of natural products appears to be a cost-effective solution. Zingiber mioga has been used as a traditional food in Asia. Recent research has reported the potential health benefits of Zingiber mioga, but the blood glucose reducing effect has not been yet evaluated.
In this study Zingiber mioga extracts (water and ethanol) were investigated for their anti-hyperglycemic and antioxidant potential using both in vitro and animal models. The in vitro study evaluated the total phenolic content, the oxygen radical absorbance capacity (ORAC) and the inhibitory effect against carbohydrate hydrolyzing enzymes (porcine pancreatic α-amylase and rat intestinal sucrase and maltase) of both Zingiber mioga extracts. Also, the extracts were evaluated for their in vivo post-prandial blood glucose reducing effect using SD rat and db/db mice models.
Our findings suggest that the ethanol extract of Zingiber mioga (ZME) exhibited the higher sucrase and maltase inhibitory activity (IC50, 3.50 and 3.13 mg/mL) and moderate α-amylase inhibitory activity (IC50, >10 mg/mL). Additionally, ZME exhibited potent peroxyl radical scavenging linked antioxidant activity (0.53/TE 1 μM). The in vivo study using SD rat and db/db mice models also showed that ZME reduces postprandial increases of blood glucose level after an oral administration of sucrose by possibly acting as an intestinal α-glucosidase inhibitor (ZME 0.1 g/kg 55.61 ± 13.24 mg/dL) CONCLUSION: The results indicate that Zingiber mioga extracts exhibited significant in vitro α-glucosidase inhibition and antioxidant activity. Additionally, the tested extracts demonstrated in vivo anti-hyperglycemic effects using SD rat and db/db mice models. Our findings provide a strong rationale for the further evaluation of Zingiber mioga for the potential to contribute as a useful dietary strategy to manage postprandial hyperglycemia.
2型糖尿病对发达国家和发展中国家而言都是一个严重问题。利用天然产物预防糖尿病前期进展为2型糖尿病似乎是一种具有成本效益的解决方案。茗荷姜在亚洲一直被用作传统食物。最近的研究报道了茗荷姜潜在的健康益处，但尚未对其降血糖作用进行评估。
在本研究中，使用体外和动物模型研究了茗荷姜提取物（水提取物和乙醇提取物）的降血糖和抗氧化潜力。体外研究评估了茗荷姜两种提取物的总酚含量、氧自由基吸收能力（ORAC）以及对碳水化合物水解酶（猪胰α-淀粉酶、大鼠肠道蔗糖酶和麦芽糖酶）的抑制作用。此外，使用SD大鼠和db/db小鼠模型评估了提取物的体内餐后降血糖作用。
我们的研究结果表明，茗荷姜乙醇提取物（ZME）表现出较高的蔗糖酶和麦芽糖酶抑制活性（IC50分别为3.50和3.13 mg/mL）以及中等的α-淀粉酶抑制活性（IC50>10 mg/mL）。此外，ZME表现出强大的过氧自由基清除相关抗氧化活性（0.53/TE 1 μM）。使用SD大鼠和db/db小鼠模型的体内研究还表明，ZME可能通过作为肠道α-葡萄糖苷酶抑制剂发挥作用，在口服蔗糖后降低餐后血糖水平的升高（ZME 0.1 g/kg时为55.61±13.24 mg/dL）。结论：结果表明，茗荷姜提取物在体外表现出显著的α-葡萄糖苷酶抑制和抗氧化活性。此外，受试提取物在使用SD大鼠和db/db小鼠模型的体内表现出降血糖作用。我们的研究结果为进一步评估茗荷姜作为控制餐后高血糖的有用饮食策略的潜力提供了有力依据。
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